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> TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

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Referência T2000-100L

Tamanho : 100L

Marca : US Biological

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Telefone : +1 850 650 7790

T2000 TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT/4°C

TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda.

TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

Appearance:
White to off white, homogenous, free flowing powder.

Solubility:
Colorless to light yellow, clear, complete.

pH:
6.5 ± 0.5

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Wang L., et al. 2018. Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) Induces Host Cell Autophagy to Benefit Infection. Viruses. 10(1), 14. doi:10.3390/v10010014|2. Dong ZQ, et al. Baculovirus LEF-11 Hijack Host ATPase ATAD3A to Promote Virus Multiplication in Bombyx mori cells. 2017. Sci Rep. 7:46187. doi: 10.1038/srep46187.|3. Gorbenko, O. et al., Monoclonal antibodies with selective specificity towards different glycosylation isoforms of FGFR1.2009) Hybridoma (Larchmt). 28(4):287-293.