TAK1-TAB1 Fusion Protein, Active
Referência T0725-13-10ug
Tamanho : 10ug
Marca : US Biological
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T0725-13 TAK1-TAB1 Fusion Protein, Active
Swiss Prot
O43318Grade
Highly PurifiedAccession #
NM_003188.2, NM_145331.1, NM_145332.1, |NM_145333.1Shipping Temp
Dry IceStorage Temp
-70°CN-terminal His6-tagged, recombinant, human TAK1 amino acids 1-303, fused to human TAB1 amino acids 437-end, expressed by baculovirus in Sf21 insect cells.
Specific Activity:
~50U/mg
Unit Definition:
One unit of TAK1-TAB1 fusion activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100uM.
Applications:
Suitable for use in MS Tryptic Fingerprint and Kinase Assay. Other applications not tested.
Recommended Dilution:
MS Tryptic Fingerprint: Confirmed identity with the translated sequence listed in the protocal.
Kinase Assay: 19-48ng phosphorylated 2mg/ml casein.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Handling Recommendations:
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.