SW480 cells
Referência C0009001
Tamanho : OneFrozenvial
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Disease:
Colorectal adenocarcinoma
Origin:
Established from a primary adenocarcinoma of the colon
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Tumorigenic; The stemline chromosome number is hypotriploid
Patient:
Male, Caucasian, 50 yrs of age
Medium:
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS
Subculture:
1:5 to 1:10 using using 0.25% trypsin or trypsin/EDTA, 0% CO2, 37°C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression:
epidermal growth factor (EGF), HLA A2, B8, B17Isoenzymes:
G6PD, B; PGM1, 2; PGM3, 1; 6PGD, A; PEP-D, 1; ES-D, 1DNA Profile (STR):
Amelogenin: X,X; CSF1PO: 13,14; D13S317: 12; D16S539: 13; D5S818: 13; D7S820: 8; THO1: 8; TPOX: 11; vWA: 16; D3S1358: 15; D21S11: 30,30.2; D18S51: 13; Penta E: 10; Penta D: 9,15; D8S1179: 13; FGA: 24Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SW480 (AddexBio C0009001)
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SW480 cells
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References for SW480 cells:
1. Hartman, J., Edvardsson, K., Lindberg, K., Zhao, C., Williams, C., Strom, A., and Gustafsson, J. A. Tumor repressive functions of estrogen receptor beta in SW480 colon cancer cells. Cancer Res, 69: 6100-6106, 2009.
2. Kong, C. K., Lam, W. S., Chiu, L. C., Ooi, V. E., Sun, S. S., and Wong, Y. S. A rice bran polyphenol, cycloartenyl ferulate, elicits apoptosis in human colorectal adenocarcinoma SW480 and sensitizes metastatic SW620 cells to TRAIL-induced apoptosis. Biochem Pharmacol, 77: 1487-1496, 2009.
3. Liao, Q. J., Su, J., He, J., Song, Y., Tang, H. L., and Su, Q. Effect of diallyl disulfide on cell cycle arrest of human colon cancer SW480 cells. Ai Zheng, 28: 138-141, 2009.
4. Shan, B. E., Wang, M. X., and Li, R. Q. Quercetin inhibit human SW480 colon cancer growth in association with inhibition of cyclin D1 and survivin expression through Wnt/beta-catenin signaling pathway. Cancer Invest, 27: 604-612, 2009.
5. Tadlaoui Hbibi, A., Laguillier, C., Souissi, I., Lesage, D., Le Coquil, S., Cao, A., Metelev, V., Baran-Marszak, F., and Fagard, R. Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide--interference with interferon-gamma-STAT1-mediated killing. Febs J, 276: 2505-2515, 2009.
6. Yavari, K., Taghikhani, M., Maragheh, M. G., Mesbah-Namin, S. A., and Babaei, M. H. Knockdown of IGF-IR by RNAi inhibits SW480 colon cancer cells growth in vitro. Arch Med Res, 40: 235-240, 2009.