Disease:

Neuroblastoma.

Origin:

SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.

Species:

Homo sapiens, human

Tissue:

Brain; derived from metastatic site: bone marrow.

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen.

Patient:

Caucasian, female, 2 years of age.

Medium:

AddexBio-formulated DMEM (C0003-01) + Non-essential amino acids (NEAA; 0.1 mM) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO