sheep Anti-lgG, H&L (Biotin) Polyclonal Antibody

Referência I1904-41N-2mg

Tamanho : 2mg

Marca : US Biological

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I1904-41N lgG, H&L (Biotin)

Clone Type
Polyclonal
Host
sheep
Source
rabbit
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Rb
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Applications:
Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.

Recommended Dilutions:
ELISA (Capture): 1:15,000-1:60,000. Coat with 1ug of Rabbit IgG.

Optimal dilutions to be determined by the researcher.

Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio:
10-20 BAC molecules per sheep IgG molecule

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: sheep|Source: rabbit|Concentration: ~2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with biotin.|Purity: Prepared by immunoaffinity chromatography followed by solid phase adsorption to remove any unwanted reactivities.|Immunogen: Rabbit IgG whole molecule|Specificity: Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum, rabbit IgG and rabbit serum.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rabbit IgG whole molecule
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with biotin.
Purity
Prepared by immunoaffinity chromatography followed by solid phase adsorption to remove any unwanted reactivities.
Specificity
Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum, rabbit IgG and rabbit serum.
References
1. Bayer & Wilchek, Methods in Enzymology 184: 138-160 (1990).