SFTPA1 ELISA Kit (Rabbit)
Referência OKCA00941
Tamanho : 96Wells
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SFTPA1 ELISA Kit (Rabbit) (OKCA00941)
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Rabbit | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology SFTPA1 ELISA Kit (Rabbit) (OKCA00941) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for SFTPA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SFTPA1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 1.56 ng/mL-100 ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level SFTPA1 were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level SFTPA1 were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.39 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | SFTPA1 |
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| Gene Full Name | Pulmonary surfactant-associated protein A |
| NCBI Gene Id | 100009044 |
| Protein Name | Pulmonary surfactant-associated protein A |
| Description of Target | In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages. |
| Uniprot ID | P12842 |
| Protein Accession # | NP_001075698.1 |
| Nucleotide Accession # | NM_001082229.1 |
