Enzyme-linked Immunoabsorbent Assay
ELISA titration assay curve of SARS-CoV-2 S polyclonal antibody (Cat# PAB31705) to SARS-CoV-2 S protein: 96-well ELISA plate was coated with SARS-CoV-2 S protein at a concentration of 2 ug/mL.
- Specifications
Product Description
Rabbit polyclonal antibody raised against full length recombinant SARS-CoV-2 S.
Immunogen
Recombinant protein corresponding to full length SARS-CoV-2 S.
Host
Rabbit
Reactivity
SARS-CoV-2
Specificity
The antibody reacts with spike protein of SARS-CoV-2. Cross-reaction to spike proteins from other coronavirus not tested.
Form
Liquid
Purification
Protein G purification
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:100-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% gelatin, 0.09% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
ELISA titration assay curve of SARS-CoV-2 S polyclonal antibody (Cat# PAB31705) to SARS-CoV-2 S protein: 96-well ELISA plate was coated with SARS-CoV-2 S protein at a concentration of 2 ug/mL.Western Blot
- Publication Reference
- In Silico and In Vitro Evaluation of the Molecular Mimicry of the SARS-CoV-2 Spike Protein by Common Short Constituent Sequences (cSCSs) in the Human Proteome: Toward Safer Epitope Design for Vaccine Development.
Yuya Mizuno, Wataru Nakasone, Morikazu Nakamura, Joji M Otaki.
Vaccines 2024 May; 12(5):539.
Application：ELISA, WB-Ti, Synthetic peptide, Human, Brain, heart, small intestine, kidney, liver, lung, skeletal mouscle, stomach, spleen, ovary, testis.
- In Silico and In Vitro Evaluation of the Molecular Mimicry of the SARS-CoV-2 Spike Protein by Common Short Constituent Sequences (cSCSs) in the Human Proteome: Toward Safer Epitope Design for Vaccine Development.