- Specifications
Product Description
Mouse monoclonal antibody raised against native RNA Polymerase II.
Immunogen
Native purified RNA Polymerase II .
Host
Mouse
Specificity
This antibody reacts with the highly conserved heptapeptide repeat of the largest subunit of eukaryotic RNA polymerase II. It inhibits promoterdirected transcription, but it does not inhibit elongation in the nonspecific transcription assay.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Purification
- Publication Reference
- Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody.
Thompson NE, Aronson DB, Burgess RR.
The Journal of Biological Chemistry 1990 Apr; 265(12):7069.
Application：AFC, IP, WB-Re, Bovine, Plant, Bovine purified proteins, Plant purified proteins.
- Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II.
Thompson NE, Steinberg TH, Aronson DB, Burgess RR.
The Journal of Biological Chemistry 1989 Jul; 264(19):11511.
Application：Dot, ELISA, Synthetic peptide.
- Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody.
RNA Polymerase II monoclonal antibody, clone 8WG16
Referência MAB8286
Tamanho : 200ug
Marca : Abnova
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