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> Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

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Referência 230-01101-100

Tamanho : 100ug

Marca : RayBiotech

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Recombinant SARS-CoV-2 Spike Protein, S1 SubunitRayBiotech's SARS-CoV-2 S1 protein induced IL-6 expression, IL-6 secretion, and STAT3 phosphorylation in brain endothelial cells. Figure adapted from Stangis et al., NeuroImmune Pharmacol. Ther., 2024.Figure adapted from Atanasova et al., Pharmaceuticals, 2024.Figure adapted from Ponce-de-León et al., npj Vaccines, 2023.RayBiotech's SARS-CoV-2 S1 protein was used to stimulate endothelial cells, triggering inflammatory responses including IL-6 (shown here) and MCP-1 upregulation. Figure adapted from Montezano et al., Scientific Reports, 2023.Figure adapted from Bejoy et al., Arch Biochem Biophys, 2023.

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit derived from E.coli
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Product Description

Specifications

Size100 µg, 250 µg
SpeciesSARS-CoV-2
Accession Number
QHD43416
Protein Name / Synonyms
SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein
Expressed Region
Val16 - Gln690
Expression SystemE. coli
TagN-terminal histidine tag
Purity
>80 %
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
~75 kDa
Format
Liquid
Formulation
Filtered solution in 50 mM Tris-HCl, 500 mM NaCl, 600 mM arginine, 2 mM oxidized glutathione, 4 mM reduced glutathione, 0.2% PEG4000, 10% glycerol, 2 mM DTT, 20 mM Hepes (pH 9).
Concentration (lot specific)
Lot specific (see the label on the vial), determined by Bradford protein assay.
Research AreaInfectious Disease, COVID-19
Shipping TypeBlue ice
Storage-20°C
SDS-PAGE Image
SDS-PAGE
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Introduction

The SARS-CoV-2 spike (S) protein is a surface glycoprotein that mediates viral entry into host cells. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for recognizing and binding to the ACE2 receptor. This interaction is the first step in viral infection, making the S1 subunit a critical focus for vaccine development and antiviral therapies.

Reference: https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03143-7

Typical Data

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) induced IL-6 expression, IL-6 secretion, and STAT3 phosphorylation in brain endothelial cells. Figure adapted from Stangis et al., NeuroImmunePharmacol. Ther., 2024.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used as an ELISA antigen to evaluate vaccine-induced IgG responses in a multi-epitope immunization study. The study also included our S2 protein (Cat# 230-01103), along with additional RayBiotech viral antigens, to assess broader humoral immunity. Figure adapted from Atanasova et al., Pharmaceuticals, 2024.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used to coat ELISA plates for detecting spike-specific IgG responses in a phase I COVID-19 vaccine trial. Shown here: IgG levels after high-dose intramuscular vaccination. Figure adapted from Ponce-de-León et al., npj Vaccines, 2023.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used to stimulate endothelial cells, triggering inflammatory responses including IL-6 (shown here) and MCP-1 upregulation. Figure adapted from Montezano et al., Scientific Reports, 2023.

RayBiotech’s recombinant spike proteins and binding assay kit were used to assess ACE2-binding inhibition by viral spike domains. Shown here: inhibition of ACE2–RBD binding by SARS-CoV-2 S1 (Cat# 230-01101), SARS-CoV-2 RBD (Cat# 230-01102), and other viral proteins using the Spike–ACE2 Binding Assay Kit I (Cat# CoV-SACE2). Figure adapted from Bejoy et al., Arch BiochemBiophys, 2023.

citations

  • A fast and easy one-step purification strategy for plant-made antibodies using Protein A magnetic beads

    Year:
    2024
    Journal Name:
    Frontiers in plant science
    PubMed ID:
    38235198

  • The S1 subunits of SARS-CoV-2 variants differentially trigger the IL-6 signaling pathway in human brain endothelial cells and downstream impact on microglia activation

    Year:
    2024
    Journal Name:
    NeuroImmune Pharm Ther
    PubMed ID:
    38532784

  • Differential Cytokine Responses of APOE3 and APOE4 Blood-brain Barrier Cell Types to SARS-CoV-2 Spike Proteins

    Year:
    2024
    Journal Name:
    J Neuroimmune Pharmacol
    PubMed ID:
    38771543

  • Design, Development and Immunogenicity Study of a Multi-Epitope Vaccine Prototype Against SARS-CoV-2

    Year:
    2024
    Journal Name:
    Pharmaceuticals (Basel)
    PubMed ID:
    39598409

  • Interim safety and immunogenicity results from an NDV-based COVID-19 vaccine phase I trial in Mexico

    Year:
    2023
    Journal Name:
    NPJ vaccines

  • SARS-CoV-2 spike protein induces endothelial inflammation via ACE2 independently of viral replication

    Year:
    2023
    Journal Name:
    Scientific reports
    PubMed ID:
    37640791

  • Effects of spike proteins on angiotensin converting enzyme 2 (ACE2)

    Year:
    2023
    Journal Name:
    Archives of Biochemistry and Biophysics
    PubMed ID:
    37769892

  • Understanding the Spike Protein in COVID-19 Vaccine in Recombinant Vesicular Stomatitis Virus (rVSV) Using Automated Capillary Western Blots

    Year:
    2023
    Journal Name:
    ACS omega

  • SARS-CoV-2 spike protein reduces burst activities in neurons measured by micro-electrode arrays

    Year:
    2023
    Journal Name:
    Annals of Medicine and Surgery

  • SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks

    Year:
    2023
    Journal Name:
    Journal of Biomedical Materials Research Part A

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Human Pulmonary Alveolar Epithelial Cells
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