Recombinant Pseudomonas aeruginosa Outer membrane porin F (oprF) - E.coli
Referência CSB-EP318417EZX-20ug
Tamanho : 20ug
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Abbreviation | Recombinant Pseudomonas aeruginosa oprF protein |
| MSDS | |
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Product Details
Purity
≥ 85% as determined by SDS-PAGE.
Target Names
oprF
Uniprot No.
Research Area
others
Alternative Names
oprF; PA1777; Outer membrane porin F
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Source
E.coli
Expression Region
25-350aa
Target Protein Sequence
QGQNSVEIEAFGKRYFTDSVRNMKNADLYGGSIGYFLTDDVELALSYGEYHDVRGTYETGNKKVHGNLTSLDAIYHFGTPGVGLRPYVSAGLAHQNITNINSDSQGRQQMTMANIGAGLKYYFTENFFAKASLDGQYGLEKRDNGHQGEWMAGLGVGFNFGGSKAAPAPEPVADVCSDSDNDGVCDNVDKCPDTPANVTVDANGCPAVAEVVRVQLDVKFDFDKSKVKENSYADIKNLADFMKQYPSTSTTVEGHTDSVGTDAYNQKLSERRANAVRDVLVNEYGVEGGRVNAVGYGESRPVADNATAEGRAINRRVEAEVEAEAK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
39.3 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend briefly centrifuging the vial prior to opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile deionized water to a recommended concentration of 0.1–1.0 mg/mL. If used immediately after reconstitution, glycerol is not required. If not for immediate use, after the protein is completely dissolved, add glycerol to a final concentration of 5%–50% (default: 50%), aliquot as needed, and store at -20℃/-80℃ for long-term storage. If dilution is needed during subsequent use, dilute with the same buffer as the protein, but without stabilizers (e.g., glycerol, trehalose, etc).
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 25-350 constitute the expression domain of recombinant Pseudomonas aeruginosa oprF. This oprF protein is theoretically predicted to have a molecular weight of 39.3 kDa. The oprF protein was expressed in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of oprF, which enables a simple process of detecting and purifying the oprF recombinant protein in the following steps.Pseudomonas aeruginosa outer membrane porin F (OprF) is a crucial component of the outer membrane in the Gram-negative bacterium Pseudomonas aeruginosa. OprF serves as a porin, forming...
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Target Background
Function
Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
Gene References into Functions
- OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b.
- LecB binds to the outer membrane protein OprF
- oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX.
- Prevention of disulfide bond formation in OprF increases its pore-forming activity.
- This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production
- Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells.
- findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated
- findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin
- analysis of OprF from Pseudomonas aeruginosa
- OprF exists in two different conformations
- analysis of Pseudomonas aeruginosa porin OprF
- Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model.
Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
OmpA family
Database Links
KEGG:
STRING: