Recombinant Human IL-15
Referência GMP-C016-1mg
Tamanho : 1mg
Marca : Novoprotein
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Description - Il-15 (Interleukin-15), is a member of the Il-2 cytokines family. Il-15 performs biological functions by γ c receptor subunits. Several therapies targeting solid tumor and blood cell tumor have entered the clinic study. Although both IL-2 and IL-15 are involved in early T cell proliferation and activation. The antitumor activity of IL-15 also comes from direct activation of CD8+ effector T cells in a non-specific antigen immunogenicity way. It is reported that there has been clinic study of IL-15 targeting immune cell therapy entering phase I.
- This product is from a large scale GMP leveled Recombinant Human Il-15 production via E. coli expression. Applying pharmaceutical leveled adjuvants and materials for production, strictly controlling host protein residues, nucleic acid residues, pathogene contamination and other impurities, we guarantee an auditable manufacture and quality control practice complying to GMP regulation, as well as all the materials traceable.
Usage In-vitro cultivation of various types of cell
Quality Control | Item | Standard |
|---|---|
| Appearance | Solidoid, white porosity |
| Reconstitution | Totally dissolve in stated time |
| Visible particle | Conform to ChP |
| pH value | 6.5-7.5 |
| Purity | ≥ 95% |
| Bioactivity | ≥ 1.0×107 IU/mg |
| Endotoxin | < 10 EU/mg |
| Host protein | ≤ 0.005% |
| Exogenous DNA residue | ≤ 10 ng/mg |
| Mycoplasma test | Negative |
| Sterility test | Conform to ChP |
Storage Storage at -20±5℃, expiration date labeled on bottle.
Recommendations - （1）Make sure the product properly stored and none expired before using.
- （2）Please use water for injection for reconstitution, to a concentration > 100 μg/ml.
- （3）Sterile condition without pyrogen is required for reconstitution, re-aliquoting, dilution.
- （4）After reconstitution, aliquoted solution vials should be stored at -70°C and minimize freeze-thaw cycles preventing denaturation of protein.
Bioactivity-Cell Based Assay 
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The specific activity of Recombinant human IL-15 is ≥ 1.0 × 107 IU/mg. which is calibrated against human IL-15 WHO International Standard (NIBSC code: 95/554)
The activity of GMP Human IL-15 Protein(Cat. No.:GMP-C016) was compared to another commercially available product.