Recombinant Human Granulocyte Colony Stimulating Factor (CHO)

Biological Activity

Human Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine, mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition it may function in some adhesion or recognition events at the cell surface. Human G-CSF is 73 % identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

Chemical Information

Form	Lyophilized powder
Solubility (25°C)	Reconstitute the lyophilized powder in distilled water to a concentration not less than 100 μg/mL.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Athanasia D Panopoulos, et al. Cytokine. Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis

[2] G D Demetri, et al. Blood. Granulocyte colony-stimulating factor and its receptor