Rat Sphingomyelin (SPH) ELISA Kit
Referência MBS265875-96
Tamanho : 96-Strip-Wells
Marca : MyBiosource
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Telefone : +1 850 650 7790
Sphingomyelin (SPH) ELISA Kit for Rat
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS265875Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat SPH monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
Sphingomyelin (SPH), ELISA Kit
Full Product Name
Rat Sphingomyelin (SPH) ELISA Kit
Product Gene Name
SPH elisa kit
Species Reactivity
Rat
Assay Type
Sandwich
Detection Range
100 ng/mL-1.56 ng/mL
Sensitivity
Up to 0.5 ng/mL
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
37,609 Da
OMIM
611575
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of SPH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SPH purchase
MBS265875 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sphingomyelin (SPH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPH. The ELISA analytical biochemical technique of the MBS265875 kit is based on SPH antibody-SPH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for SPH. It may not necessarily be applicable to this product.
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sphingomyelin synthase-related protein 1 isoform 1
NCBI Official Synonym Full Names
sterile alpha motif domain containing 8
NCBI Official Symbol
SAMD8
NCBI Official Synonym Symbols
SMSr; HEL-177
UniProt Protein Name
Sphingomyelin synthase-related protein 1
UniProt Synonym Protein Names
Ceramide phosphoethanolamine synthase; CPE synthase; Sterile alpha motif domain-containing protein 8; SAM domain-containing protein 8
Protein Family
Sphingomyelin phosphodiesterase
UniProt Gene Name
SAMD8
UniProt Synonym Gene Names
SMSr; CPE synthase; SAM domain-containing protein 8
UniProt Entry Name
SAMD8_HUMAN
NCBI Protein Information
sphingomyelin synthase-related protein 1; CPE synthase; epididymis luminal protein 177; sphingomyelin synthase related; SAM domain-containing protein 8; ceramide phosphoethanolamine synthase; sterile alpha motif domain-containing protein 8
UniProt Comments for SPH
SAMD8: Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway. Belongs to the sphingomyelin synthase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10q22.2
Cellular Component: integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: transferase activity
Biological Process: ceramide biosynthetic process; sphingomyelin biosynthetic process
Show more Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10q22.2
Cellular Component: integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: transferase activity
Biological Process: ceramide biosynthetic process; sphingomyelin biosynthetic process
Research Articles on SPH
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with SPH elisa kit
| Reference Product | PubMed Publications |
|---|---|
| ANKS3 elisa kit | >1 publications with SPH and ANKS3 |
Pathways associated with SPH elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Sphingomyelin Metabolism Pathway antibodies | Sphingomyelin Metabolism Pathway Diagram |
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