Rat IgG Antibody : AP
Referência OASB01916
Tamanho : 1.0mL
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
Goat Anti-Rat IgG Antibody - AP Conjugated (OASB01916)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rat IgG Antibody - AP Conjugated (OASB01916) |
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| Tested Species Reactivity | Rat |
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| Predicted Species Reactivity | Rat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | AP |
| Application | IHC-F, ICC, WB |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with rat IgG; purified by affinity chromatography on pooled rat IgG covalently linked to agarose; reacts with the heavy chain of rat IgG; cross-adsorbed against rat IgM for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgG |
| Purification | Affinity chromatography on rat IgG covalently linked to agarose |
| Concentration | Lot specific |
| Specificity | IgG |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: FITC, and TRITC conjugates BIOT conjugate R-PE conjugate AP conjugate HRP conjugate BGAL conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 1:500 1:5,000-1:20,000 |
| Application Info | Direct immunofluorescent staining of IgG+ rat B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3 Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The Beta- galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS/NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Rat IgM |
| Gene Full Name | Ig gamma-1 chain C region |
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