Rat Free Fatty Acid (FFA) ELISA Kit
Referência MBS266907-96
Tamanho : 96-Strip-Wells
Marca : MyBiosource
Free Fatty Acid (FFA) ELISA Kit for Rat
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS266907Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat FFA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
Free Fatty Acid (FFA), ELISA Kit
Full Product Name
Rat Free Fatty Acid (FFA) ELISA Kit
Product Gene Name
FFA elisa kit
Species Reactivity
Rat
Assay Type
Sandwich
Detection Range
10 mmol/L-0.156 mmol/L
Sensitivity
Up to 0.05 mmol/L
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
37,144 Da
OMIM
603823
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of FFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FFA purchase
MBS266907 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Free Fatty Acid (FFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FFA. The ELISA analytical biochemical technique of the MBS266907 kit is based on FFA antibody-FFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for FFA. It may not necessarily be applicable to this product.
NCBI Official Full Name
free fatty acid receptor 2
NCBI Official Synonym Full Names
free fatty acid receptor 2
NCBI Official Symbol
FFAR2
NCBI Official Synonym Symbols
FFA2R; GPR43
UniProt Protein Name
Free fatty acid receptor 2
UniProt Synonym Protein Names
G-protein coupled receptor 43
UniProt Gene Name
FFAR2
UniProt Synonym Gene Names
GPR43
UniProt Entry Name
FFAR2_HUMAN
NCBI Protein Information
free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; free fatty acid activated receptor 2
NCBI Summary for FFA
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]
Show more UniProt Comments for FFA
FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: cell projection; integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding
Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; positive regulation of cytokine production during immune response; cell surface pattern recognition receptor signaling pathway; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production
Show more Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: cell projection; integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding
Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; positive regulation of cytokine production during immune response; cell surface pattern recognition receptor signaling pathway; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production
Research Articles on FFA
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with FFA elisa kit
| Reference Product | PubMed Publications |
|---|---|
| FFAR1 elisa kit | >6 publications with FFA and FFAR1 |
| CCK elisa kit | >1 publications with FFA and CCK |
Pathways associated with FFA elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| Free Fatty Acid Receptors Pathway antibodies | Free Fatty Acid Receptors Pathway Diagram |
| G Alpha (q) Signalling Events Pathway antibodies | G Alpha (q) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
| GPCR Ligand Binding Pathway antibodies | GPCR Ligand Binding Pathway Diagram |
| GPCRs, Class A Rhodopsin-like Pathway antibodies | GPCRs, Class A Rhodopsin-like Pathway Diagram |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies | Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram |
| Signal Transduction Pathway antibodies | Signal Transduction Pathway Diagram |
| Signaling By GPCR Pathway antibodies | Signaling By GPCR Pathway Diagram |
| CAMP Signaling Pathway antibodies | CAMP Signaling Pathway Diagram |
Diseases associated with FFA elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >8 publications with FFA and Inflammation |
| Colitis Antibodies | >5 publications with FFA and Colitis |
| Fatty Liver Antibodies | >4 publications with FFA and Fatty Liver |
| Liver Diseases Antibodies | >2 publications with FFA and Liver Diseases |
| Hyperglycemia Antibodies | >2 publications with FFA and Hyperglycemia |
| Disease Models, Animal Antibodies | >2 publications with FFA and Disease Models, Animal |
| Asthma Antibodies | >1 publications with FFA and Asthma |
| Nervous System Diseases Antibodies | >1 publications with FFA and Nervous System Diseases |
| Hypersensitivity Antibodies | >1 publications with FFA and Hypersensitivity |
| Weight Loss Antibodies | >1 publications with FFA and Weight Loss |
Organs/Tissues associated with FFA elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Lung Antibodies | >1 publications with FFA and Lung |
| Pancreas Antibodies | >1 publications with FFA and Pancreas |
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