Rat Cytokine Array Q282 (Glass Slide)

Referência QAR-CAA-282-1

Tamanho : 8SampleKit

Marca : RayBiotech

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Telefone : +1 850 650 7790

Rat Cytokine Array Q282

Quantibody® Rat Cytokine Array 282 is a combination of 8 non-overlapping arrays (QAR-CYT-3,4,5,6,7,8,9, and QAR-SAP-1) to quantitatively measure the concentration of 282 rat cytokines. Suitable for all liquid sample types.

Full testing services and biostats analysis available:

Quantitative Proteomics Services

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Product Description

Specifications

Size8 Sample Kit, 22 Sample Kit, 50 Sample Kit
SpeciesRat
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected282
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaAntibody Drug Development, Biomarker Detection
Shipping TypeBlue ice
Storage-20°C

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), ACE-2, Activin A, Activin R2A, ADAM-9, ADAMTS-1 (METH1), Adiponectin (ACRP30), aFGF (FGF-1), ALCAM (CD166), Ameloblastin, Angiopoietin-2, Angiotensinogen (SERPINA8), ApoH, ASAH2 (Neutral Ceramidase), BAFF, BAMBI (NMA), BDNF, Beta-2 Microglobulin, beta-NGF, BID, BMP-7, BST1, C4.4A (LYPD3), Cadherin-4, Carbonic Anhydrase XIV (CA14), Carboxypeptidase A1 (CPA1), Carboxypeptidase B1 (CPB1), Cathepsin B (Pro-Cathepsin B), Cathepsin C, Cathepsin E, Cathepsin L, CD14, CD155 (PVR), CD164, CD1d1, CD226, CD28, CD300LG, CD34, CD36 (SR-B3), CD38, CD40 (TNFRSF5), CD44 (Heparan Sulfate Proteoglycan), CD47, CD48 (SLAMF2), CD5, CD59, CD5L, CD6, CD80 (B7-1), CD83, CD86 (B7-2), CD93, CDH5, CDNF, CEACAM-1, CINC-1 (CXCL1), CINC-2, CINC-3, CLEC4B2, CLEC5A, Clusterin, CNTF, Coagulation Factor III (Tissue Factor/Thromboplastin), Coagulation Factor XIV (Protein C), Contactin-1, Contactin-3 (BIG-1), Contactin-4 (BIG-2), COPZ1, CPA2, CRELD1, CRELD2, CRP (C-Reactive Protein), CSF1R, CTACK (CCL27), CXCL16, Cystatin C, Decorin, DKK-3, DLL1, DSC2, ECM-1, EGF, EGFR, Endoglin (CD105), Eotaxin-1 (CCL11), EphA3, EphA5, EphB3, EphB6, Ephrin-A2, ErbB3, Erythropoietin, E-Selectin, FABP4 (A-FABP), Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), FCAR (CD89), FCGR3A, FCGRT, FETUB, FGF R4, FGF-12, FGF-21, FGF-BP, Flt-3 Ligand, Follistatin, Follistatin-like 1, FOLR1, Fractalkine (CX3CL1), Galectin-1, Galectin-3, Gas 1, GDF-3, GDF-8 (Myostatin), GDNF, GFR alpha-1 (GDNF R), GFR alpha-2, GFR alpha-3, GGT1, GITR (TNFRSF18), GM-CSF, gp130 (sgp130), Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3), HGF, HPX, Human Amyloid-like protein 1 (APLP-1), ICAM-1 (CD54), Iduronate 2-Sulfatase (IDS), IFN alpha 5, IFN-gamma, IFN-gamma R2, IGF-1, IGFBP-1, IGSF4B (SynCAM3/CADM3), IGSF8 (CD316), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-11 R alpha, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15 R alpha, IL-17 RA, IL-17 RC, IL-17F, IL-18, IL-2, IL-2 R alpha, IL-2 R gamma (Common gamma Chain), IL-20 RB, IL-21, IL-21 R, IL-22, IL-3, IL-31, IL-4, IL-4R, IL-5, IL-6, IL-7, IL-7 R, IL-9, IL-9 R (CD129), Jagged 1, JAM-A (CD321/F11R), JAM-C (CD323), KDR, Kininogen (Kininostatin), KLKB1, Klotho beta, Lactoferrin, Layilin, Legumain, Leptin, LIF, Lipocalin-2 (NGAL), LIX, LRPAP, L-Selectin (CD62L), Lumican, Lymphotoxin beta R (TNF RIII), MAG, Matrilin-4, MBL-2, MCP-1 (CCL2), MCP-3 (MARC/CCL7), MESDC2, MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MMP-2, MSP alpha/beta, Nephrin, Neurexin 1 alpha, Neurexophilin-1, Neurexophilin-3, Neuroligin-1 (NLGN1), Neuropilin-1, Neuropilin-2, Neuroplastin, Noggin, Nope, Notch-1, Notch-2, Notch-3, NPC2, NT5E, Olfactomedin-1, Osteopontin (SPP1), OX40 (TNFRSF4), P-Cadherin, PDGF R alpha, PDGF-AA, PDGF-BB, Pentraxin-2, PGC, PGRPs, pIgR, Plexin A1, PLTP, Pref-1 (DLK-1/FA1), PROCR, Prolactin, Prolactin R, Protocadherin-12, PSAP, P-Selectin, PVRL1, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Reg3A, Reg3B, Reg4, Renin 1, RGM-B, ROBO1, SCF, SCGF (CLEC11a), SEMA3F, Semaphorin 4D, Semaphorin 7A, SERPINA1c, SERPINA3N, SERPIND1 (Heparin Cofactor II), SERPINE1, SERPINF1 (PEDF), SERPINF2 (Alpha-2-Antiplasmin), SGSH, SIGNR1 (CD209b), SIRP alpha, Sortilin, SP-D, STX7, Syndecan-1, Syndecan-3, TCK-1 (CXCL7), TGF beta R3, TIM-1 (KIM-1), TIMP-1, TIMP-2, TNF alpha, Transferrin, TREM-1, TrkA, TRKB, TROP1 (EpCAM), Trypsin 3 (PRSS3), TWEAK R (TNFRSF12), UCH-L1, UNC5H1 (Netrin Receptor UNC5A), VEGF-A, VEGFR1, XPNPEP2

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Rat Cytokine Array Q282 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktails
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAR-CYT-3.gal)
GAL (GAL-QAR-CYT-4.gal)
GAL (GAL-QAR-CYT-5.gal)
GAL (GAL-QAR-CYT-6.gal)
GAL (GAL-QAR-CYT-7.gal)
GAL (GAL-QAR-CYT-8.gal)
GAL (GAL-QAR-CYT-9.gal)
GAL (GAL-QAR-SAP-1.gal)
Excel (QAR-CYT-3_Analysis_Tool.xls)
Excel (QAR-CYT-4_Analysis_Tool.xls)
Excel (QAR-CYT-5_Analysis_Tool.xls)
Excel (QAR-CYT-6_Analysis_Tool.xls)
Excel (QAR-CYT-7_Analysis_Tool.xls)
Excel (QAR-CYT-8_Analysis_Tool.xls)
Excel (QAR-CYT-9_Analysis_Tool.xls)
Excel (QAR-SAP-1_Analysis_Tool.xls)

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