Rabbit IgG F(ab')2 Antibody F(ab')2 - AP Conjugated
Referência OARA03863
Tamanho : 500µg
Marca : Aviva Systems Biology
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Goat Anti-Rabbit IgG F(ab')2 Polyclonal Antibody - AP Conjugated (OARA03863)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rabbit IgG F(ab')2 Polyclonal Antibody - AP Conjugated (OARA03863) |
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| Predicted Species Reactivity | Rabbit |
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| Product Format | Liquid. 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugation | AP |
| Application | ELISA|IHC|WB |
| Additional Information | Conjugation Chemistry: Glutaraldehyde coupling Color: Substrate dependent |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Rabbit IgG F(ab')2 fragment |
| Purification | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
| Concentration | 0.5 mg/ml |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins. |
| Application Info | ELISA: 1:5000 IHC: 1:200~1000 WB: 1:500~2500 F(ab')2 Anti-Rabbit IgG F(ab')2 Alkaline Phosphatase Antibody has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Alias Symbols | ALP, Alk Phos |
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| Description of Target | F(ab')2 Anti-Rabbit IgG F(ab')2 Alkaline Phosphatase Antibody generated in goat detects Rabbit F(ab')2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. |
| Molecular Weight | 140 kDa |