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> Rabbit Anti-TARDBP Polyclonal Antibody

Rabbit Anti-TARDBP Polyclonal Antibody

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Referência HPA017284-25ul

Tamanho : 25ul

Marca : Atlas Antibodies

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HPA017284
TAR DNA binding protein

Anti-TARDBP Antibody

Polyclonal Antibody against HUMAN TARDBP

0.05 mg/ml
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
Anti-TARDBP Antibody
info@quimigen.pt

Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Product details

  • Target protein: TAR DNA binding protein
  • Target gene: TARDBP
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000041459 (96%)RAT - ENSRNOG00000012455 (32%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000067632
  • Concentration: 0.05 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:20 - 1:50
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100

Validation

  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 
  • Immunofluorescence in Cell Lines (ICC-IF) 

Target information

  • Protein name: TAR DNA binding protein
  • Gene name: TARDBP
  • Alternative gene names: ALS10, TDP-43, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (5)

  • Sapaly D, Delers P, Coridon J, Salman B, Letourneur F, Dumont F, Lefebvre SFront Mol Biosci , 2020 Apr 17; 7:55. Epub 2020 Apr 172020 Apr 17
  • Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg ENat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 242013 Apr

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