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> Rabbit Anti-SOX10 Polyclonal Antibody

Rabbit Anti-SOX10 Polyclonal Antibody

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Referência HPA068898

Tamanho : 100ul

Marca : Atlas Antibodies

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Telefone : +1 850 650 7790

HPA068898
SRY (sex determining region Y)-box 10

Anti-SOX10 Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN SOX10

0.1 mg/ml
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
Anti-SOX10 Antibody
info@quimigen.pt

Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA068898 antibody. Corresponding SOX10 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: SRY (sex determining region Y)-box 10
  • Target gene: SOX10
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000033006 (98%)RAT - ENSRNOG00000011305 (98%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000049962
  • Concentration: 0.1 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:500 - 1:1000
    • Retrieval method: HIER pH6

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  

Target information

  • Protein name: SRY (sex determining region Y)-box 10
  • Gene name: SOX10
  • Alternative gene names: DOM, WS2E, WS4, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (8)

  • Montarolo F, Berrino L, Rominto AM, Loddo M, Balbo I, Ricci A, Servetto GP, Mango G, Yamamoto K, Parolisi R, Bertolotto A, Vigliaturo R, Menotta M, Zha S, Tempia F, Hoxha EActa Neuropathol Commun , 2026 May 29; 14(1). Epub 2026 May 292026 May 29
  • Rajan AAN, Hutchins EJbioRxiv , . Epub 2026 Feb 32026 Feb 3