Rabbit Anti-PBRM1 Polyclonal Antibody

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Telefone : +1 850 650 7790

HPA059373

polybromo 1

Anti-PBRM1 Antibody
- ChIP-Certified

Polyclonal Antibody against HUMAN PBRM1

0.2 mg/ml

info@quimigen.pt

Western blot analysis using Anti-PBRM1 antibody HPA059373 (A) shows similar pattern to independent antibody HPA015629 (B).

Target protein: polybromo 1
Target gene: PBRM1
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000042323 (100%)RAT - ENSRNOG00000028227 (100%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Chromatin Immunoprecipitation (ChIP) recommended conditions
- Working concentration: 1-10µg per reaction
Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications.
Immunofluorescence in Cell Lines (ICC-IF)
Western Blot (WB)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas