Rabbit Anti-ONECUT2 Polyclonal Antibody

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Telefone : +1 850 650 7790

HPA057058

one cut homeobox 2

Anti-ONECUT2 Antibody
- ChIP-Certified

Polyclonal Antibody against HUMAN ONECUT2

0.2 mg/ml

info@quimigen.pt

Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.

Target protein: one cut homeobox 2
Target gene: ONECUT2
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000045991 (100%)RAT - ENSRNOG00000052665 (100%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Chromatin Immunoprecipitation (ChIP) recommended conditions
- Working concentration: 1-10µg per reaction
Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100

Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications.
Immunofluorescence in Cell Lines (ICC-IF)

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Gutiérrez M, Zamora I, Iriarte R, Pajares MJ, Yang Q, Qian C, Otegui N, Fernández-Irigoyen J, Santamaría E, Alcala N, Sexton-Oates A, Fernández-Cuesta L, Barajas M, Calvo A, Montuenga LM, Knudsen B, You S, Freeman MR, Encío I, Rotinen MMol Med , 2025 Jun 11; 31:232. Epub 2025 Jun 112025 Jun 11
Qian C, Yang Q, Rotinen M, Huang R, Kim H, Gallent B, Yan Y, Cadaneanu RM, Zhang B, Kaochar S, Freedland SJ, Posadas EM, Ellis L, Di Vizio D, Morrissey C, Nelson PS, Brady L, Murali R, Campbell MJ, Yang W, Knudsen BS, Mostaghel EA, Ye H, Garraway IP, You S, Freeman MRNucleic Acids Res , 2024 Jun 27; 52(13):7740-7760. Epub 2024 Jun 272024 Jun 27