rabbit Anti-Integrin, alpha5 (ITGA5, FNRA, Integrin alpha-5, CD49 Antigen-like Family Member E, Fibronectin Receptor Subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 Heavy Chain, Integrin alpha-5 Light Chain) Monoclonal Antibody [Clone:D7B7G]

Integrins are a/B heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 alpha and 8 B subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

Integrin alpha5/B1 is involved in multiple biological processes including embryonic development, angiogenesis and tumor metastasis (4,5). By interaction with its fibronectin ligand, alpha5/ B1 transduces signals that regulate cell adhesion, migration, matrix assembly and cytoskeletal organization (6).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.