Rabbit Anti-DNPEP Polyclonal Antibody

Imprimir

Referência HPA044860

Tamanho : 100ul

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Atlas Antibodies

Solicitar mais informações

Contactar o distribuidor local :

Telefone : +1 850 650 7790

HPA044860

aspartyl aminopeptidase

Anti-DNPEP Antibody

Polyclonal Antibody against HUMAN DNPEP

0.1 mg/ml

info@quimigen.pt

Immunohistochemical staining of human cerebral cortex, lymph node, pancreas and testis using Anti-DNPEP antibody HPA044860 (A) shows similar protein distribution across tissues to independent antibody HPA036398 (B).

Product details

Target protein: aspartyl aminopeptidase
Target gene: DNPEP
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000026209 (92%)RAT - ENSRNOG00000019772 (91%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: R41153
Concentration: 0.1 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: aspartyl aminopeptidase
Gene name: DNPEP
Alternative gene names: ASPEP, DAP,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas