Rabbit Anti-BICD2 Polyclonal Antibody

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Referência HPA023013

Tamanho : 100ul

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Telefone : +1 850 650 7790

Marca : Atlas Antibodies

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

HPA023013

bicaudal D homolog 2 (Drosophila)

Anti-BICD2 Antibody

Polyclonal Antibody against HUMAN BICD2

0.2 mg/ml

info@quimigen.pt

Immunohistochemistry analysis in human skin and liver tissues using HPA023013 antibody. Corresponding BICD2 RNA-seq data are presented for the same tissues.

Product details

Target protein: bicaudal D homolog 2 (Drosophila)
Target gene: BICD2
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000037933 (100%)RAT - ENSRNOG00000016031 (100%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000070003
Concentration: 0.2 mg/ml

Applications

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100

Validation

Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)
Immunofluorescence in Cell Lines (ICC-IF)

Target information

Protein name: bicaudal D homolog 2 (Drosophila)
Gene name: BICD2
Alternative gene names: KIAA0699,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (5)

Leong EL, Khaing NT, Cadot B, Hong WL, Kozlov S, Werner H, Wong ES, Stewart CL, Burke B, Lee YLHum Mol Genet , 2022 Aug 4; 32(2):177-191. Epub 2022 Aug 42022 Aug 4
Agote‐Aran A, Schmucker S, Jerabkova K, Jmel Boyer I, Berto A, Pacini L, Ronchi P, Kleiss C, Guerard L, Schwab Y, Moine H, Mandel J, Jacquemont S, Bagni C, Sumara IEMBO J , 2020 Jul 24; 39(20):e104467. Epub 2020 Jul 242020 Jul 24