PSIP1 ELISA Kit (Chicken)
Referência OKEH06657
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
PSIP1 ELISA Kit (Chicken) (OKEH06657)
| Datasheets/Manuals | Printable datasheet for PSIP1 ELISA Kit (Chicken) (OKEH06657) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Psip1 ELISA Kit (Chicken) (OKEH06657) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Psip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psip1 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50ng/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.27 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Lens epithelium-derived growth factor | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | PSIP1 |
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| Gene Full Name | PC4 and SFRS1 interacting protein 1 |
| Alias Symbols | lens epithelium-derived growth factor, PSIP2. |
| NCBI Gene Id | 431605 |
| Protein Name | Lens epithelium-derived growth factor |
| Description of Target | Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis (By similarity). |
| Uniprot ID | Q5XXA9 |
| Protein Accession # | NP_001026781 |
| Nucleotide Accession # | NM_001031610 |
