PCK2 ELISA Kit (Chicken)
Referência OKEH06622
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
PCK2 ELISA Kit (Chicken) (OKEH06622)
| Datasheets/Manuals | Printable datasheet for PCK2 ELISA Kit (Chicken) (OKEH06622) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Pck2 ELISA Kit (Chicken) (OKEH06622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pck2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pck2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pck2 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50ng/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.17 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Phosphoenolpyruvate carboxykinase [GTP], mitochondrial | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | PCK2 |
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| Gene Full Name | phosphoenolpyruvate carboxykinase 2, mitochondrial |
| Alias Symbols | PEPCK-M, phosphoenolpyruvate carboxykinase [GTP], mitochondrial, phosphoenolpyruvate carboxylase. |
| NCBI Gene Id | 396457 |
| Protein Name | Phosphoenolpyruvate carboxykinase [GTP], mitochondrial |
| Description of Target | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Facilitates the recycling of lactate carbon in the liver. |
| Uniprot ID | P21642 |
| Protein Accession # | NP_990801 |
| Nucleotide Accession # | NM_205470 |
