PC-PLD2 (phospho Tyr169) Polyclonal Antibody

Product Name :
PC-PLD2 (phospho Tyr169) Polyclonal Antibody Background :
phospholipase D2(PLD2) Homo sapiens The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011], Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Phospho-PC-PLD2 (Y169) Polyclonal Antibody detects endogenous levels of PC-PLD2 protein only when phosphorylated at Y169. Immunogen :
The antiserum was produced against synthesized peptide derived from human PLD2 around the phosphorylation site of Tyr169. AA range:136-185 Conjugate :
Unconjugated Modification :
Phosphorylation

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  Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLD2 (Phospho-Tyr169) Antibody
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  Immunohistochemistry analysis of paraffin-embedded human brain, using PLD2 (Phospho-Tyr169) Antibody. The picture on the right is blocked with the phospho peptide.
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  Immunohistochemistry analysis of paraffin-embedded human brain, using PLD2 (Phospho-Tyr169) Antibody. The picture on the right is blocked with the phospho peptide.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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