Passage Reagent Group™ is a matched set of Cell Systems Certified™ reagents (3x100mL) for releasing cells from culture for subculture or freezing.
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- PRODUCT INFO
- CITATIONS
- DOCUMENTATION
Gain Consistency with our Single Lot Media and Reagents
This PRG Group™ is qualified for use with all Cell Systems Certified Mediums. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™(Trypsin Inhibitor-dPBS Solution) each in a 100mL bottle.
Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.
PRG-1™ (EDTA-dPBS Solution): The chelating agent EDTA in PRG-1™ prepares the cells for PRG-2™ processing.
PRG-2™: Contains highly purified trypsin, allowing a substantial reduction (40%) in the amount of enzyme required to detach cells compared to typical commercial trypsin solutions. The trypsin in PRG-2™ is inactivated by trypsin inhibitors in the PRG-3™ formulation, preventing nonspecific protease damage to cell membranes after detachment.
PRG-3™: Inactivates the protease in PRG-2™ and stabilizes the cell membranes for passaging and freezing. PRG-3™ is engineered to work together with Attachment Factor™ (4Z0-210) to stabilize the cell membrane and promote spreading and the establishment of correct polarity after subculture.
Our Passage Reagent System™ (4Z0-840), adds AttachmentFactor™ to the PRG Group™.
Storage and Handling
Store at +2 - 8°C. Once opened, shelf life 30 days at +2 - 8°C.
Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classified "Sterile".
A Selection of Citations for 4Z0-800 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems reagents.
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"Optimization of an in vitro human blood–brain barrier model: Application to blood monocyte transmigration assays" Paradis et al. MethodsX, 2016
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"Shiga Toxin Mediated Neurologic Changes in Murine Model of Disease" Pradhan et al. Frontiers in Cellular and Infection Microbiology, 2016
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"Effect of endoplasmic reticulum stress mediated by hyperglycemia on barrier function in retinal cells" Saif (Dissertation) 2016
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"Adenosine Kinase Inhibition Protects The Kidney Against Streptozotocin-Induced Diabetes Through Anti-inflammatory and Anti-oxidant" Mechanisms" Pye et al. Pharmacological Research, 2015
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"S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis" Abu El-Asrar et al. Molecular Vision, 2014)
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"Differential expression and role of hyperglycemia induced oxidative stress in epigenetic regulation of β1, β2 and β3-adrenergic receptors in retinal endothelial cells" Safi et al. BMC Medical Genomics, 2014
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"Optical Recording Reveals Novel Properties of GSK1016790A-Induced Vanilloid Transient Receptor Potential Channel TRPV4 Activity in Primary Human Endothelial Cells" Sullivan et al. Molecular Pharmacology, 2012
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"High-Glucose-Induced Endothelial Cell Injury Is Inhibited by a Peptide Derived from Human Apolipoprotein E." Bhattacharjee et al. PLoS One, 2012
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"Soluble aggregates of the amyloid-beta are trapped by serum albumin to enhance amyloid-beta activation of endothelial cells" Moss et al. Journal of Biological Engineering, 2009