N-Phenylacrylamide [2210-24-4]

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Referência NB-64-45648-1mL

Tamanho : 1mLx10mM(inDMSO)

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Telefone : +1 850 650 7790

N-Phenylacrylamide

Catalog No. T9322 Copy Product Info

Purity: 99.99%

N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay.

N-Phenylacrylamide

Copy Product Info

N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay.

Cas No. 2210-24-4

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Batch Information

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Purity:99.99%

Appearance:solid

Color:White

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COA LCMS HNMR

Product Introduction

N-Phenylacrylamide AI Summary

N-Phenylacrylamide is a bioactive inhibitor of human recombinant Transglutaminase 2 (TG2), with an IC50 value of 43000.0 nM based on fluorescent transamidation assay results. It exhibits various pharmacokinetic properties, including a half-life of 4.983 hours in phosphate buffer at pH 7.4 at a concentration of 50 uM in the presence of glutathione. It shows intrinsic clearance rates in human and rat hepatocytes of approximately 30.9 and 32.36 uL/min/(10^6 cells), respectively, indicating moderate metabolism and elimination in liver cells. Additionally, N-Phenylacrylamide inhibits several enzymes—such as West Nile virus NS2B-NS3 protease, Dengue virus serotype 2 NS2B-NS3 protease, Bovine thrombin, human and Escherichia coli MetAP, and wild-type Escherichia coli Mur enzymes—with inhibition rates of less than 15.0%. It also inhibits human FBPase by 17.0% at 500 uM. Furthermore, the compound demonstrates GSH reactivity and forms adducts, evident from RP-HPLC analysis, and shows a pseudo-first-order reaction with GSH at 37°C (K = 13.0 x 10^-3/min) with a half-life of 0.88 hours for GSH reactivity. In terms of thiol reactivity, N-Phenylacrylamide has a pseudo-first-order rate constant of 1.6 x 10^-4/s in a PBS/DMSO buffer environment. When tested against cysteine in PBS buffer using NMR assay, it has a half-life of 2.3 hours and a rate constant of 8.38 x 10^-5/s. Additionally, it shows a half-life of 4.75 hours in GSH reactivity assessment using DNTB and exhibits a Gibbs free energy change of 24.1 kcal/mol at 1 uM concentration within 120 minutes, with a pseudo-first-order rate constant of 3.87 x 10^-3/min and a half-life of 2.983 hours under pH 7.4 phosphate buffer conditions. N-Phenylacrylamide also manifests cytotoxicity against HeLa cells, with an EC50 greater than 100,000.0 nM, further highlighting its potential bioactivity and reactivity in various biological contexts..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay.
Targets&IC50	TGase 2:43 µM

Chemical Properties

Molecular Weight	147.17
Formula	C₉H₉NO
Cas No.	2210-24-4
Smiles	C=CC(=O)Nc1ccccc1
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (373.72 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.7949 mL	33.9743 mL	67.9486 mL	339.7432 mL
5 mM	1.3590 mL	6.7949 mL	13.5897 mL	67.9486 mL
10 mM	0.6795 mL	3.3974 mL	6.7949 mL	33.9743 mL
20 mM	0.3397 mL	1.6987 mL	3.3974 mL	16.9872 mL
50 mM	0.1359 mL	0.6795 mL	1.3590 mL	6.7949 mL
100 mM	0.0679 mL	0.3397 mL	0.6795 mL	3.3974 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.