ELM-TNFa-1 | Mouse TNF-alpha ELISA Kit Quimigen Portugal

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Mouse TNF-alpha ELISA Kit Standard Curve

Mouse TNF-alpha ELISA Kit

RayBio^® Mouse TNF alpha ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Sensitivity	60 pg/ml (sensitivity of lysate is 94 pg/ml) Need more sensitivity? Check out the new NexaTag^® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	60 pg/ml - 6000 pg/ml (detection range of lysate is 94 pg/ml - 6000 pg/ml)
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)	2 fold
Accession Number	P06804
Gene Id	21926
Gene Symbols	TNF, TNFSF2
Protein Name / Synonyms	Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	≤-20°C

Target Background

Tumor necrosis factor alpha (TNF alpha), also known as lymphotoxin and cachectin, is a pleiotropic cytokine. TNF alpha is a smaller cytokine with a molecular weight of 26 kDa (transmembrane) and 17 kDa (soluble) that binds to the same receptors producing a vast array of effects similar to those of TNF-beta. Both TNF-alpha and TNF-beta share 30% amino acid homology and have similar biological activities. TNF-alpha is produced primarily by monocytes, neutrophils and macrophages. It is found in synovial cells and macrophages in the tissues. TNF-alpha and TNF-beta can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 (55 kDa) and 2 (75 kDa) to be killed, with the p55 receptor mediating the cytotoxic response.

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

RayBiotech’s ELM-TNFα, ELM-MCP1, ELM-IL6, and ELM-IL1β ELISA kits were used to measure serum TNF-α, MCP-1, IL-6, and IL-1β in high-fat diet–fed adipocyte-specific TRPM7 knockout mice. Figure adapted from Zhong et al., Nature Communications, 2023.

RayBiotech’s ELM-TNFα, ELM-IFNγ, ELM-IL1β, ELM-IL17, ELM-IL6, and ELM-IL10 ELISA kits were used to measure TNF-α, IFN-γ, IL-1β, IL-17A, IL-6, and IL-10 in colon tissue homogenates from DSS-induced colitis mice with or without white kidney bean supplementation. Figure adapted from Sun et al., Journal of Translational Medicine, 2025.

RayBiotech’s ELM-TNFα, ELM-IL4, ELM-IL6, ELM-IL10, ELM-IL1β, and ELM-IL17 ELISA kits were used to measure serum TNF-α, IL-4, IL-6, IL-10, IL-1β, and IL-17a in DSS-induced colitis mice treated with Shaoyao decoction. Figure adapted from Wang et al., Journal of Ethnopharmacology, 2025.

RayBiotech’s ELM-TNFa-CL-1, ELM-IFN-γ-CL-1, and ELM-IL1β-CL-1 ELISA kits were used to measure TNF-α, IFN-γ, and IL-1β levels in matched retinal and plasma samples from male and female mice following systemic LPS administration. Figure adapted from Rodríguez-Ramírez et al., Frontiers in Immunology, 2024.

RayBiotech’s ELM-TNFα and ELM-IL6 ELISA kits were used to measure TNF-α and IL-6 levels in UVB-irradiated mouse skin. Figure adapted from Kim et al., Redox Biology, 2023.

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Additional Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µL to 1 µL volumes
Adjustable 1-25 µL pipettes for reagent preparation
100 µL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µL of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

documents

Datasheet (ELM-TNFa.pdf)

Datasheet (ELM-TNFa-CL.pdf)

SDS (ELM-TNFa.pdf)

SDS (ELM-TNFa-CL.pdf)

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

Adipose-specific deletion of the cation channel TRPM7 inhibits TAK1 kinase-dependent inflammation and obesity in male mice
Species:
Mouse
Sample Type:
Serum
Year:
2023
Journal Name:
Nature Communications
Oral Microalgae-Nano Integrated System against Radiation-Induced Injury
Species:
Mouse
Sample Type:
small intestinal tissue
Year:
2023
Journal Name:
Acs Nano
WKB ameliorates DSS-induced colitis through inhibiting enteric glial cells activation and altering the intestinal microbiota
Year:
2025
Journal Name:
Journal of Translational Medicine
PubMed ID:
39838431
Shaoyao decoction alleviates DSS-induced colitis by inhibiting IL-17a-mediated polarization of M1 macrophages
Year:
2025
Journal Name:
Journal of Ethnopharmacology
PubMed ID:
39427735
Retinal response to systemic inflammation differs between sexes and neurons
Year:
2024
Journal Name:
Frontiers in immunology
PubMed ID:
38384465
Possible pathogenic mechanisms for doxorubicin-induced splenic atrophy in a human breast cancer xenograft mouse model
Year:
2024
Journal Name:
Journal of Applied Toxicology
PubMed ID:
38943348
Metformin instigates cellular autophagy to ameliorate high-fat diet-induced pancreatic inflammation and fibrosis/EMT in mice
Year:
2024
Journal Name:
Biochim Biophys Acta Mol Basis Dis
PubMed ID:
38901652
Anatomical eModule Curriculum: Perceptions of Undergraduate Medical Students Amidst Limited Experiential Laboratory Learning
Year:
2024
Journal Name:
Med Sci Educ
PubMed ID:
39450021
Protective effects of an electrophilic metabolite of docosahexaenoic acid on UVB-induced oxidative cell death, dermatitis, and carcinogenesis
Species:
Mouse
Sample Type:
skin tissue
Year:
2023
Journal Name:
Redox biology
T follicular helper cells contribute to pathophysiology in a model of neuromyelitis optica spectrum disorders
Year:
2023
Journal Name:
JCI insight
PubMed ID:
36649074

Mouse TNF-alpha ELISA Kit

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Mouse TNF-alpha ELISA Kit