Mouse Klotho (KL) ELISA Kit
Referência MBS2022324-96
Tamanho : 96-Strip-Wells
Marca : MyBiosource
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Klotho (KL) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Popular Catalog #: MBS2022324SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to klotho. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to klotho. Next, Avidin… More details
Product Overview
Online Datasheet in PDFProduct Name
Klotho (KL), ELISA Kit
Full Product Name
Mouse Klotho (KL) ELISA Kit
Product Gene Name
KL elisa kit
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Mouse serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.12-200pg/mL
Sensitivity
< 1.24pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of klotho. No significant cross-reactivity or interference between klotho and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level klotho were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level klotho were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
62,135 Da
OMIM
211900
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for KL elisa kit
Metabolic pathway
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of KL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KL purchase
MBS2022324 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Klotho (KL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KL. The ELISA analytical biochemical technique of the MBS2022324 kit is based on KL antibody-KL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
klotho
NCBI Official Symbol
KL
UniProt Protein Name
Klotho
Protein Family
Klotho
UniProt Gene Name
KL
UniProt Entry Name
KLOT_HUMAN
NCBI Protein Information
klotho
NCBI Summary for KL
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for KL
Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Membrane protein, integral; EC 3.2.1.31
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region
Molecular Function: vitamin D binding; signal transducer activity; beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; hormone activity; fibroblast growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging
Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial
Show more Protein type: Hydrolase; Membrane protein, integral; EC 3.2.1.31
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region
Molecular Function: vitamin D binding; signal transducer activity; beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; hormone activity; fibroblast growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging
Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial
Research Articles on KL
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with KL elisa kit
| Reference Product | PubMed Publications |
|---|---|
| FGF23 elisa kit | >39 publications with KL and FGF23 |
Pathways associated with KL elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies | Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram |
| DAP12 Interactions Pathway antibodies | DAP12 Interactions Pathway Diagram |
| DAP12 Signaling Pathway antibodies | DAP12 Signaling Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| Downstream Signal Transduction Pathway antibodies | Downstream Signal Transduction Pathway Diagram |
| Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies | Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram |
| Downstream Signaling Of Activated FGFR Pathway antibodies | Downstream Signaling Of Activated FGFR Pathway Diagram |
| Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies | Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram |
| Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies | Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram |
Diseases associated with KL elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Pneumonia Antibodies | >212 publications with KL and Pneumonia |
| Fibrosis Antibodies | >149 publications with KL and Fibrosis |
| Skin Diseases Antibodies | >127 publications with KL and Skin Diseases |
| Pulmonary Fibrosis Antibodies | >120 publications with KL and Pulmonary Fibrosis |
| Inflammation Antibodies | >89 publications with KL and Inflammation |
| Heart Diseases Antibodies | >73 publications with KL and Heart Diseases |
| Kidney Diseases Antibodies | >61 publications with KL and Kidney Diseases |
| Lung Injury Antibodies | >60 publications with KL and Lung Injury |
| Necrosis Antibodies | >46 publications with KL and Necrosis |
| Hypertension Antibodies | >40 publications with KL and Hypertension |
Organs/Tissues associated with KL elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Bone Antibodies | >297 publications with KL and Bone |
| Brain Antibodies | >238 publications with KL and Brain |
| Kidney Antibodies | >129 publications with KL and Kidney |
| Connective Tissue Antibodies | >68 publications with KL and Connective Tissue |
| Eye Antibodies | >38 publications with KL and Eye |
| Spleen Antibodies | >35 publications with KL and Spleen |
| Intestine Antibodies | >23 publications with KL and Intestine |
| Parathyroid Antibodies | >18 publications with KL and Parathyroid |
| Pancreas Antibodies | >14 publications with KL and Pancreas |
| Ganglia Antibodies | >13 publications with KL and Ganglia |
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