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> Mouse Anti-Rabbit IgG - HRP For IP/CoIP

Mouse Anti-Rabbit IgG - HRP For IP/CoIP

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Referência CSA9108-500ul

Tamanho : 500ul

Marca : Cohesion Biosciences

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  • Immunoprecipitating GAPDH in Hela whole cell lysate with rabbit anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Rabbit IgG - HRP and Anti-Rabbit IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Rabbit IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.
Product Name:Anti-Rabbit IgG - HRP For IP/CoIP
Cat No:CSA9108
Source:
Reactivity:Rb
Applications:IP, CoIP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Monoclonal Secondary Antibody to Rabbit IgG HRP labeled
Immunogen：Rabbit IgG
Purification：The antibody is isolated from cell culture by protein A/G immunoaffinity chromatography.
Specificity：The antibody is a conformation-specific secondary antibody that only reacts with native rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured IgG light and heavy chains of the primary antibody used for IP will not be recognised.
Clonality：Monoclonal
Conjugation：HRP
Form：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% Proclin
Dilution：WB (1/500 - 1/2000)
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.