Mouse Anti-MBP Monoclonal Antibody [Clone:CL2829]

Referência AMAb91064-25ul

Tamanho : 25ul

Marca : Atlas Antibodies

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AMAb91064
myelin basic protein

Anti-MBP Antibody

Monoclonal Antibody against HUMAN MBP

1.0 mg/ml
info@quimigen.pt

Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb91064 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.

Product details

  • Target protein: myelin basic protein
  • Target gene: MBP
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000041607 (97%)RAT - ENSRNOG00000016516 (97%)
  • Clonality: Monoclonal
  • Clone ID: CL2829
  • Isotype: IgG1
  • Host: Mouse
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-03963
  • Concentration: 1.0 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:5000 - 1:10000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml

Validation

  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: myelin basic protein
  • Gene name: MBP
  • Ensembl: 
  • UniProt: 
  • Entrez: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (6)

  • Schäffner E, Bosch-Queralt M, Edgar JM, Lehning M, Strauß J, Fleischer N, Kungl T, Wieghofer P, Berghoff SA, Reinert T, Krueger M, Morawski M, Möbius W, Barrantes-Freer A, Stieler J, Sun T, Saher G, Schwab MH, Wrede C, Frosch M, Prinz M, Reich DS, Flügel A, Stadelmann C, Fledrich R, Nave KA, Stassart RMNat Neurosci , 2023 Jun 29; 26(7):1218-1228. Epub 2023 Jun 292023 Jun 29

  • Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön MBMC Biol , 2023 May 24; 21:113. Epub 2023 May 242023 May 24

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