mouse Anti-CD8 (CD 8, CD8 Antigen alpha Polypeptide, Leu2 T Lymphocyte Antigen, MAL, OKT8 T Cell Antigen, p32, T Cell Antigen Leu2, T Cell Surface Glycoprotein CD8 alpha Chain) (FITC) Monoclonal Antibody [Clone:CT6]

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Referência C2259-12F1-100T

Tamanho : 100Tests

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Telefone : +1 850 650 7790

Marca : US Biological

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

C2259-12F1 CD8 (CD 8, CD8 Antigen alpha Polypeptide, Leu2 T Lymphocyte Antigen, MAL, OKT8 T Cell Antigen, p32, T Cell Antigen Leu2, T Cell Surface Glycoprotein CD8 alpha Chain) (FITC)

Clone Type

Monoclonal

Host

mouse

Source

guinea pig

Isotype

IgG1

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CT6|Host: mouse|Source: guinea pig|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography. |Immunogen: Guinea pig peritoneal T-cells.|Specificity: Recognizes an antigen present on a subset of guinea pig T-lymphocyte with the same distribution as CD8 positive T-cells in man and mouse.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Guinea pig peritoneal T-cells.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes an antigen present on a subset of guinea pig T-lymphocyte with the same distribution as CD8 positive T-cells in man and mouse.

References

1. Tan, B.T.G. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening. Hybridoma 4: 115-124.|2. Steerenberg, P.A. et al. (1991) Tumour rejection after transfer of line 10 immunity is mediated by two T-cell populations. Cancer Immunol. Immunother. 34: 103-110.3. Steerenberg, P.A. et al. (1990) Tumour infiltrating leucocytes (tils) during progressive tumour growth and BCG - mediated tumour regression. progressive tumour growth and BCG - mediated tumour regression. Virchows Archiv Cell Pathol. 59: 185-194.4. Baker, D. et al. (1987) Changes in lymphocyte subsets after treatment with cyclophosphamide and during the development of contact sensitivity in the guinea pig. Int. J. Immunophamacol. 9: 175-183.5. Antoniou, A.V. et al. (1986) Immunocytochemical identification and quantitation of mononuclear cells in the meninges during the development of Chronic Relapsing Experimental Allergic Encephalomyelitis (CREAE) in the guinea pig. Cell. Immunol. 97: 386-396.7. Liversidge, J. and Forrester, J.V. (1988) Experimental autoimmune uveitis (EAU): Immunophenotypic analysis of inflammatory cells in chorio retinal lesions. Curr. Eye Res. 7: 1231-1240.8. Debout, C. et al. (1991) The Kurloff Cell in Estrogenized guinea pigs as a CT7+ 8BE6-CT6-MR-1-CT10-IgM-lymphocyte with natural killer activity. Nat. Immun. Cell Growth Regul. 10: 327-335.