Monoclonal Rat anti-Mouse CD3E Antibody (clone YCD3-1, FITC, IHC)
Referência LS-C21539-100
Tamanho : 100ug
Marca : LSBio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Overview
Antibody:
CD3E Rat anti-Mouse Monoclonal (FITC) (YCD3-1) Antibody
Application:
IHC, IP, Flo
Reactivity:
Mouse
Format:
FITC, Unmodified
Specifications
Description
CD3E antibody LS-C21539 is an FITC-conjugated rat monoclonal antibody to mouse CD3E. Validated for Flow, IHC and IP.
Target
Mouse CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
YCD3-1
Conjugations
FITC. Also available conjugated with FITC, APC, PE, Cy5, FITC, Biotin, PE, APC, PE, Cy5, Cy5, Biotin, PE, APC, PE, Cy5, FITC, Biotin, PE, PE, Cy5, FITC, Biotin, PE, FITC, Biotin, PE, APC, PE, Cy5, Cy5, PE, Cy5.5, PE, Cy7, Biotin.
Purification
Purified
Modifications
Unmodified
Specificity
Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
Applications
- IHC
- Immunoprecipitation
- Flow Cytometry
- Applications tested for the base form of this product only
Usage
Identification and enumeration of CD3+ cells by flow cytometry. Immunoprecipitation. In vitro depletion of CD3+ cells. In vitro activation of T cells (accessory cells must be provided). Immunohistochemistry tested with mouse samples fixed in PLP and then paraffin-embedded (see Whiteland etal (1995) J Histochem Cytochem 43:313). Flow Cytometry: 3 ug/1E6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee About CD3E
NM_000733 NP_000724.1
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@quimigen.pt