Monkey Interleukin-18(IL18) ELISA Kit
Referência CSB-E08979Mk-5x96T
Tamanho : 5x96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 94 | ||||
Range % | 86-99 | |||||
1:2 | Average % | 92 | ||||
Range % | 88-98 | |||||
1:4 | Average % | 101 | ||||
Range % | 94-114 | |||||
1:8 | Average % | 99 | ||||
Range % | 92-104 | |||||
The recovery of monkey IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 99 | 92-103 | ||||
EDTA plasma (n=4) | 90 | 85-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
 | ||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||
3000 | 3.659 | 3.672 | 3.666 | 3.517 | ||
1500 | 2.148 | 2.158 | 2.153 | 2.005 | ||
750 | 1.283 | 1.306 | 1.294 | 1.146 | ||
375 | 0.756 | 0.765 | 0.760 | 0.612 | ||
187.5 | 0.474 | 0.470 | 0.472 | 0.324 | ||
94 | 0.312 | 0.328 | 0.320 | 0.172 | ||
47 | 0.241 | 0.241 | 0.241 | 0.093 | ||
0 | 0.148 | 0.149 | 0.148 |
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