Monkey Interleukin 17A (IL-17A/IL-17) ELISA Kit

Referência CSB-E08905Mk-24T

Tamanho : 24T

Marca : Cusabio

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Telefone : +1 850 650 7790

CSB-E08905Mk
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 17A
Associated names
Interleukin-17A elisa kit;IL-17 elisa kit; IL-17A elisa kit
Abbreviation
IL-17A/IL-17
Uniprot No.
Species
Monkey
Sample Types
serum, plasma, cell culture supernates
Detection Range
9.38 pg/mL-600 pg/mL.
Sensitivity
4.69 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-17A/IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

89

Range %

82-92

1:2

Average %

93

Range %

89-99

1:4

Average %

98

Range %

91-103

1:8

Average %

104

Range %

97-111

Recovery

The recovery of monkey IL-17A/IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

95

89-98

EDTA plasma (n=4)

90

82-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

600

3.598

3.664

3.631

3.567

300

2.771

2.895

2.833

2.768

150

1.650

1.680

1.665

1.601

75

0.826

0.861

0.843

0.779

37.5

0.473

0.500

0.486

0.422

18.75

0.224

0.221

0.223

0.158

9.38

0.158

0.161

0.159

0.095

0

0.063

0.066

0.065

 

ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months

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