Monkey Interleukin 17A (IL-17A/IL-17) ELISA Kit
Referência CSB-E08905Mk-24T
Tamanho : 24T
Marca : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-17A/IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 89 | ||||
Range % | 82-92 | |||||
1:2 | Average % | 93 | ||||
Range % | 89-99 | |||||
1:4 | Average % | 98 | ||||
Range % | 91-103 | |||||
1:8 | Average % | 104 | ||||
Range % | 97-111 | |||||
The recovery of monkey IL-17A/IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 90 | 82-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
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pg/ml | OD1 | OD2 | Average | Corrected | ||
600 | 3.598 | 3.664 | 3.631 | 3.567 | ||
300 | 2.771 | 2.895 | 2.833 | 2.768 | ||
150 | 1.650 | 1.680 | 1.665 | 1.601 | ||
75 | 0.826 | 0.861 | 0.843 | 0.779 | ||
37.5 | 0.473 | 0.500 | 0.486 | 0.422 | ||
18.75 | 0.224 | 0.221 | 0.223 | 0.158 | ||
9.38 | 0.158 | 0.161 | 0.159 | 0.095 | ||
0 | 0.063 | 0.066 | 0.065 |
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