A4233-1ml | Methylprednisolone [83-43-2] Quimigen Portugal

Background

Methylprednisolone is a synthetic glucocorticoid receptor agonist, used to achieve prompt suppression of inflammation.

In vitro: Methylprednisolone (2-10 mg/kg) significantly inhibited TNF production. High doses of methylprednisolone (50 mg/kg) increased LPS-induced IL-10 levels. Methylprednisolone (0.01-100 μg/ml) increased the biosynthesis of IL-10 in LPS-activated mouse peritoneal macrophages [1]. In WG patients and controls, methylprednisolone (MP) down-regulated the spontaneous and the staphylococcal enterotoxin B (SEB)-induced release of chemokines from peripheral blood mononuclear cells (PBMC)[2]. Treatment with 0.25 mM methylprednisolone inhibited acantholysis in skin cultures directly [3].

In vivo: Methylprednisolone (30 mg/kg, i.v.) given immediately after SCI reduced TNF-α expression by 55% (P<0.01) and NF-kB binding activity. Methylprednisolone suppressed the Post-traumatic inflammatory activity caused by TNF-alpha-NF-kB cascade[4]. Intravenously administration of MP (30 mg/kg) reduced the number of ED1-positive cells by 82% in the rostral cord stump and 66% in the caudal stump. In the adult rat, MP administration shortly after spinal cord transection resulted in a long-term reduction of ED1-positive cells and spinal tissue loss, reduced dieback of vestibule spinal fibres, and a transient sprouting of vestibule spinal fibres near the lesion at 1 and 2 weeks post-lesion. MP treatment also significantly reduced the tissue loss in both cord stumps at 2, 4 and 8 week post-injury[5].

Cllinical trials: In patients with acute spinal cord injury diagnosed in National Acute Spinal Cord Injury Study (NASCIS) centers within 8 hours of injury, methylprednisolone treatment for 48 hours improved motor recovery at 6 weeks (P= 0.09) and 6 months (P= 0.07) after injury[6]. In patients with acute spinal-cord injury, methylprednisolone (30 mg/kg) followed by infusion at 5.4 mg/kg/hour for 23 hours improved neurologic recovery. Among patients treated with methylprednisolone (30 mg/kg), mortality at 14 days was significantly increasedsecondary infection[7]. Methylprednisolone has entered clinical trials in patients with severe renel vasculitis and lupus nephritis.

References:
[1] Marchant A, Amraoui Z, Gueydan C, et al. Methylprednisolone differentially regulates IL‐10 and tumour necrosis factor (TNF) production during murine endotoxaemia[J]. Clinical & Experimental Immunology, 1996, 106(1): 91-96.
[2] Torheim E A, Yndestad A, Bjerkeli V, et al. Increased expression of chemokines in patients with Wegener's granulomatosis modulating effects of methylprednisolone in vitro[J]. Clinical & Experimental Immunology, 2005, 140(2): 376-383.
[3] Swanson D L, Dahl M V. Methylprednisolone inhibits pemphigus acantholysis in skin cultures[J]. Journal of investigative dermatology, 1983, 81(3): 258-260.
[4] Xu J, Fan G, Chen S, et al. Methylprednisolone inhibition of TNF-α expression and NF-kB activation after spinal cord injury in rats[J]. Molecular brain research, 1998, 59(2): 135-142.
[5] Oudega M, Vargas C G, Weber A B, et al. [J]. European Journal of Neuroscience, 1999, 11(7): 2453-2464.Long‐term effects of methylprednisolone following transection of adult rat spinal cord
[6] Bracken M B, Shepard M J, Holford T R, et al. Administration of methylprednisolone for 24 or 48 hours or tirilazadmesylate for 48 hours in the treatment of acute spinal cord injury: results of the Third National Acute Spinal Cord Injury Randomized Controlled Trial[J]. Jama, 1997, 277(20): 1597-1604.
[7] Bracken M B, Shepard M J, Collins W F, et al. A randomized, controlled trial of methylprednisolone or naloxone in the treatment of acute spinal-cord injury: results of the Second National Acute Spinal Cord Injury Study[J]. New England Journal of Medicine, 1990, 322(20): 1405-1411.

Chemical Properties

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	374.48
Cas No.	83-43-2
Formula	C₂₂H₃₀O₅
Solubility	insoluble in H2O; ≥15.35 mg/mL in DMSO; ≥9.5 mg/mL in EtOH with ultrasonic
Chemical Name	(6S,8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-6,10,13-trimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopentaamphenanthren-3-one
SDF	Download SDF
Canonical SMILES	CC1CC2C3CCC(C3(CC(C2C4(C1=CC(=O)C=C4)C)O)C)(C(=O)CO)O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Chemical structure

Methylprednisolone [83-43-2]

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Background

Chemical Properties

Quality Control

Chemical structure