Leptin tA Protein
Referência OPPA00618
Tamanho : 10µg
Marca : Aviva Systems Biology
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Leptin tA Protein (OPPA00618)
| Datasheets/Manuals | Printable datasheet for Leptin tA Protein (OPPA00618) |
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| Predicted Species Reactivity | Rat |
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| Product Format | The protein was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3. Physical appearance: White lyophilized (freeze-dried) powder. |
| Host | E. Coli |
| Additional Information | Solubility: It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Rat Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted with other aqueous solutions. |
| :: | Biological Activity: Leptin Antagonist Triple Mutant Rat Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. |
| :: | Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| :: | Product Description: Leptin Antagonist Triple Mutant Rat Recombinant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Rat Recombinant was purified by proprietary chromatographic techniques. |
| Reconstitution and Storage | Lyophilized Leptin-Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0. 1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP-tA mutant can be stored at 4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. |
| Purity | Greater than 99.0% as determined by (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. |
| Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Val - Pro - Ile - Gln. |
| Gene Symbol | rLeptin tA |
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| Alias Symbols | Leptin tA Rat, Leptin Antagonist Triple Mutant Rat Recombinant |
| Description of Target | Recombinant Rat Leptin Triple Antagonist |
| Protein Size (# AA) | Recombinant |