IL-11, Recombinant, Human, Animal Free (Interleukin-11, AGIF)

Interleukin-11 (IL-11) is a potent lymphoid cell growth factor which stimulates the growth and survivability of certain B cells and T cells. Human IL-11 is a 19.1kD protein containing 178 amino acid residues.

Source:
Recombinant protein corresponding to human IL-11 expressed in E. coli.

Biological Activity:
Determined by its ability to stimulate the proliferation of mouse T11 cells. The expected ED50 is <2.0ng/ml, corresponding to a specific activity of >5x10e5units/mg.

Endotoxin:
<0.1ng/ug of protein (<1EU/ug)

AA Sequence:
MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL
RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD
LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL
QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG
LHLTLDWAVR GLLLLKTRL

Protein Content:
Verified by UV Spectroscopy and/or SDS-PAGE gel.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.