IgG ELISA Kit (Rat)
Referência OKIA00153
Tamanho : 1x96wellplate
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
IgG ELISA Kit (Rat) (OKIA00153)
| Datasheets/Manuals | Printable datasheet for IgG ELISA Kit (Rat) (OKIA00153) |
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| Predicted Species Reactivity | Rat | ||||||||||||||||
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| Product Format | In Solution | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||
| ELISA Kit Duration | 100 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology IgG ELISA Kit (Rat) (OKIA00153) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgG - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG captured in the well. | ||||||||||||||||
| ELISA Kit Range | 25 ng/ml - 800 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The IgG test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in biological samples of rats. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Rat IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-RAT IgG ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat IgG. 7. RAT IgG CALIBRATOR One vial containing Rat IgG calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 3.620 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Rat | ||||||||||||||||
| Publications | Quantitative temporal analysis of pancreatic islet T lymphocyte and macrophage infiltration heralded by serum IgE in congenic BioBreeding (BB) Gimap5-/- rats at risk for insulitis and acute onset diabetes | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Alias Symbols | IGHG1, Ig gamma, Immunoglobulin G, Ig gamma-1 chain C region |
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| Protein Name | IgG |