ICAM1 ELISA Kit (Bovine)
Referência OKEH07248
Tamanho : 96T
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
ICAM1 ELISA Kit (Bovine) (OKEH07248)
| Datasheets/Manuals | Printable datasheet for ICAM1 ELISA Kit (Bovine) (OKEH07248) |
|---|
| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ICAM1 ELISA Kit (Bovine) (OKEH07248) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ICAM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ICAM1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=6.7% (n = 20) Mean Inter-assay CV%: <=10.4% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Additional Information | Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. | ||||||||||||||||||||||
| :: | Subcellular Location: Membrane Single-pass type I membrane protein | ||||||||||||||||||||||
| :: | Unigene: Bt.5372 SMR: Q95132 String: 9913.ENSBTAP00000013608 | ||||||||||||||||||||||
| :: | KEGG: bta:281839 Pfam: PF03921 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 0.15ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Intercellular adhesion molecule 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | ICAM1 |
|---|---|
| Gene Full Name | intercellular adhesion molecule 1 |
| Alias Symbols | ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor. |
| NCBI Gene Id | 281839 |
| Protein Name | Intercellular adhesion molecule 1 |
| Description of Target | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). |
| Uniprot ID | Q95132 |
| Protein Accession # | NP_776773.1 |
| Nucleotide Accession # | NM_174348.2 |