Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Human Thrombospondin 1 ELISA Kit
Human Thrombospondin 1 ELISA Kit
RayBio® Human Thrombospondin 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 6 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 6 ng/ml - 2000 ng/ml |
| Specificity | This ELISA antibody pair detects human Thrombospondin-1. It shows less than 5% cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 10 - 200 fold |
| Accession Number | P07996 |
| Gene Id | 7057 |
| Gene Symbols | THBS1, TSP, TSP1 |
| Protein Name / Synonyms | Thrombospondin-1 |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human Thrombospondin 1 qIPCR ELISA Kit
As low as $749.00
RayPlex® Human Thrombospondin 1 Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Thrombospondin 1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Thrombospondin-1 (TSP-1 / THBS1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
TCOF1 affects Golgi secretory pathway contributing to the angiogenesis in renal cancer
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media from ccRCC cells)
- Year:
- 2026
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 41840584
Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (fibroblasts with THBS1 knockdown/overexpression)
- Year:
- 2024
- Journal Name:
- International Journal of Oral Science
- PubMed ID:
- 38403794
Mdm2-mediated neddylation of pVHL blocks the induction of antiangiogenic factors
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media of H1299, 786-O, RCC4 cells)
- Year:
- 2020
- Journal Name:
- Oncogene
- PubMed ID:
- 32555333
THBS1 Is a Novel Serum Prognostic Factors of Acute Myeloid Leukemia
- Species:
- Human
- Sample Type:
- Serum (AML patients vs healthy controls)
- Year:
- 2019
- Journal Name:
- Frontiers in Oncology
- PubMed ID:
- 32117788
Evaluating von Willebrand factor and ADAMTS13 levels in thalassemia major patients and assessing a possible association with Thrombospondin-1
- Species:
- Human
- Sample Type:
- blood
- Year:
- 2023
- Journal Name:
- International Journal of Laboratory Hematology
