Human soluble fibrin monomer complex,SFMC ELISA Kit
Referência CSB-E09097h-24T
Tamanho : 24T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
soluble fibrin monomer complex,SFMC
Abbreviation
SFMC
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine
Detection Range
6.25 μg/mL-400 μg/mL
Sensitivity
1.56 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
Recovery
| The recovery of human SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-91 | ||||
| EDTA plasma (n=4) | 99 | 95-103 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human SFMC ELISA Kit was designed for the quantitative measurement of Human SFMC protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 6.25 μg/mL-400 μg/mL and the sensitivity is 1.56 μg/mL.
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■ Q&A
Q:
I want to know the antibody pair used for this kit.
A:
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CSB-E09097h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
CSB-E09097h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.