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Human PDGF-AB ELISA Kit
Human PDGF-AB ELISA Kit
RayBio® Human PDGF-AB ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 10 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 10 pg/ml - 2500 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF-BB, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P01127 |
| Gene Id | 5155 |
| Gene Symbols | PDGFB, SIS, PDGF2 |
| Protein Name / Synonyms | Platelet-derived growth factor subunit B (PDGF subunit B) (PDGF-2) (Platelet-derived growth factor B chain) (Platelet-derived growth factor beta polypeptide) (Proto-oncogene c-Sis) (Becaplermin) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human PDGF-AB qIPCR ELISA Kit
As low as $749.00
RayPlex® Human PDGF-AB Bead Assay
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human PDGF-AB ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified PDGF-AB, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
The HCG11/QKI5 Axis Regulates Endothelial Angiogenic Adaptation During Chronic Cerebral Hypoperfusion
- Species:
- Human
- Sample Type:
- Plasma (chronic cerebral hypoperfusion patients)
- Year:
- 2026
- Journal Name:
- CNS Neuroscience & Therapeutics
- PubMed ID:
- 42484275
Effects of acetazolamide combined with remote ischemic preconditioning on risk of acute mountain sickness: a randomized clinical trial
- Species:
- Human
- Sample Type:
- Plasma (all trial participants)
- Year:
- 2024
- Journal Name:
- BMC Medicine
- PubMed ID:
- 38166913
Assessment Of Growth Factors Released From Partially Demineralized Tooth Matrix Based On Donor Age And Gender
- Species:
- Human
- Sample Type:
- Other / Unspecified (partially demineralized tooth matrix)
- Year:
- 2024
- Journal Name:
- African Journal of Biomedical Research
Comparing the release of growth factors when partially demineralized tooth matrix is mixed with platelet rich fibrin and injectable platelet rich fibrin: An in-vitro observational study
- Species:
- Human
- Sample Type:
- Other / Unspecified (PRF, i-PRF, platelet concentrates combined with PDTM)
- Year:
- 2024
- Journal Name:
- Journal of oral and maxillofacial pathology : JOMFP
- PubMed ID:
- 39670126
Helpful to Live Healthier? Intermittent Hypoxic/Ischemic Training Benefits Vascular Homeostasis and Lipid Metabolism with Activating SIRT1 Pathways in Overweight/Obese Individuals
- Species:
- Human
- Sample Type:
- Serum (overweight/obese individuals)
- Year:
- 2024
- Journal Name:
- Obesity facts
- PubMed ID:
- 38185107
GSDMB is increased in IBD and regulates epithelial restitution/repair independent of pyroptosis
- Species:
- Human
- Year:
- 2022
Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis
- Species:
- Human
- Sample Type:
- Serum (blood-derived preparations human sera)
- Year:
- 2020
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 32854428
Conditioned Medium Originated From Lethally Irradiated Umbilical Cord Blood-Derived Mononuclear Cells Has Different Pro-Angiogenic Effects Over Mature and Progenitor Endothelial Cells In Vitro.
- Species:
- Human
- Sample Type:
- Conditioned Media (monnuclear cells)
- Year:
- 2013
- Journal Name:
- Blood
Enhancement of Human Cancer Cell Motility and Invasiveness by Anaphylatoxin C5a via Aberrantly Expressed C5a Receptor (CD88)
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2013
- Journal Name:
- Clinical Cancer Research
Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation
- Species:
- Human
- Sample Type:
- Tissue Lysate (dHACM, dehydrated Human Amnion/chorion membrane)
- Year:
- 2014
- Journal Name:
- Journal of Biomedical Materials Research Part B: Applied Biomaterials
- PubMed ID:
- 24664953
