Human Kappa, Mouse ads Antibody : PE
Referência OASB01869
Tamanho : 0.25mg
Marca : Aviva Systems Biology
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Goat Anti-Human Kappa Antibody - PE Conjugated (OASB01869)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Human Kappa Antibody - PE Conjugated (OASB01869) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | R-PE |
| Application | FLISA, FC |
| Additional Information | Description: Pepsin digest of Goat Anti-Human Kappa, Mouse ads - Pooled antisera from goats hyperimmunized with human kappa light chains; purified by affinity chromatography on human kappa light chains covalently linked to agarose; reacts with human kappa light chains; cross-adsorbed against human lambda light chains / mouse immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Human kappa light chains |
| Purification | Affinity chromatography on pooled human Igs with- - light chains covalently linkedto agarose. |
| Concentration | 0.25 mg/mL |
| Specificity | Kappa |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence ELISA: FITC conjugate BIOT conjugate R-PE conjugate BIOT conjugate AP conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:5,000-1:20,000 1:2,000-1:4,000 |
| Application Info | Direct immunofluorescence staining, B cell enumeration, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Pooled human myeloma proteins with light chains and mouse immunoglobulins |