Human IL-3 Protein, His tag (Animal-Free)
Referência MBS9719488-0,005mg
Tamanho : 0.005mg
Marca : MyBiosource
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IL-3 Human Active Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9719488Interleukin-3 (IL-3) is a pleiotropic cytokine which can stimulates the survival, differentiation and proliferation of committed progenitor cells, including megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. IL-3… More details
Product Overview
Online Datasheet in PDFProduct Name
IL-3, Active Protein
Full Product Name
Human IL-3 Protein, His tag (Animal-Free)
Product Gene Name
IL-3 active protein
Product Synonym Names
MCGF (Mast Cell Growth Factor); Multi-CSF; HCGF; P-cell stimulation factor; Interleukin-3b
Sequence
MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIFwithpo lyhistidin etagattheC -terminus.
Host
E coli
Form/Format
The protein was lyophilized from a 0.2 um filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity/Purification
>98% as determined by SDS-PAGE
Preparation and Storage
Lyophilized protein should be stored at -20 degree C for 1 year. Upon reconstitution, store at 2 degree C to 8 degree C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degree C or -80 degree C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight
17,233 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (131396347..131398896). Location: 5q31.1
OMIM
147740
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Endotoxin
<0.1 EU per 1 ug of the protein by the LAL method.
Activity
Measured by its ability to induce TF-1 cells proliferation. The ED?? for this effect is <0.15 ng/mL. The specific activity of recombinant human IL-3 is approximately >1.2 x 10^6 IU/mg.
Usage Notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 Protein, His tag (Animal-Free) using the buffer we provided not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Product Categories/Family for IL-3 active protein
Cytokines and proteins; Cytology; Cell Research Tools; Cell Culture
Other Notes
Small volumes of IL-3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL-3 purchase
MBS9719488 is an active protein targeting IL-3. Also known as Human IL-3 Protein, His tag (Animal-Free). Host: E coli.
Additional Details
NCBI/Uniprot data below describe general gene information for IL-3. It may not necessarily be applicable to this product.
NCBI Official Full Name
IL-3
NCBI Official Synonym Full Names
interleukin 3 (colony-stimulating factor, multiple)
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MULTI-CSF
UniProt Protein Name
Interleukin-3
UniProt Synonym Protein Names
Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
UniProt Gene Name
IL3
UniProt Synonym Gene Names
IL-3; MCGF
UniProt Entry Name
IL3_HUMAN
NCBI Protein Information
interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
NCBI Summary for IL-3
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for IL-3
IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.
Protein type: Cytokine; Oncoprotein; Apoptosis; Secreted; Secreted, signal peptide; Cell cycle regulation
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity
Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; cell-cell signaling; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication
Show more Protein type: Cytokine; Oncoprotein; Apoptosis; Secreted; Secreted, signal peptide; Cell cycle regulation
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity
Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; cell-cell signaling; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with IL-3 active protein
| Reference Product | PubMed Publications |
|---|---|
| IL2 active protein | >80 publications with IL-3 and IL2 |
| IL5 active protein | >48 publications with IL-3 and IL5 |
| JAK2 active protein | >13 publications with IL-3 and JAK2 |
| CSF2RB active protein | >10 publications with IL-3 and CSF2RB |
| IL7 active protein | >8 publications with IL-3 and IL7 |
| FOS active protein | >7 publications with IL-3 and FOS |
| IL3RA active protein | >7 publications with IL-3 and IL3RA |
| GRB2 active protein | >2 publications with IL-3 and GRB2 |
| PIK3CA active protein | >1 publications with IL-3 and PIK3CA |
Pathways associated with IL-3 active protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Asthma Pathway antibodies | Asthma Pathway Diagram |
| Asthma Pathway antibodies | Asthma Pathway Diagram |
| Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies | Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram |
| Calcium Signaling In The CD4+ TCR Pathway antibodies | Calcium Signaling In The CD4+ TCR Pathway Diagram |
| Cytokine Signaling In Immune System Pathway antibodies | Cytokine Signaling In Immune System Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Fc Epsilon RI Signaling Pathway antibodies | Fc Epsilon RI Signaling Pathway Diagram |
Diseases associated with IL-3 active protein
| Disease Name | Pubmed Publications |
|---|---|
| Hematologic Diseases Antibodies | >49 publications with IL-3 and Hematologic Diseases |
| Bone Marrow Diseases Antibodies | >37 publications with IL-3 and Bone Marrow Diseases |
| Necrosis Antibodies | >27 publications with IL-3 and Necrosis |
| Disease Models, Animal Antibodies | >25 publications with IL-3 and Disease Models, Animal |
| Lung Diseases Antibodies | >20 publications with IL-3 and Lung Diseases |
| Inflammation Antibodies | >17 publications with IL-3 and Inflammation |
| Drug Toxicity Antibodies | >17 publications with IL-3 and Drug Toxicity |
| Skin Diseases Antibodies | >16 publications with IL-3 and Skin Diseases |
| Asthma Antibodies | >14 publications with IL-3 and Asthma |
| Anemia Antibodies | >12 publications with IL-3 and Anemia |
Organs/Tissues associated with IL-3 active protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Mixed Antibodies | >11 publications with IL-3 and Mixed |