This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HCV-Ab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HCV-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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| 96T | Inquiry |
- Product Name
- Human HCV IgG(Hepatitis C Virus Antibody IgG) ELISA Kit
- Alias
- HCV antibody ELISA Kit, Hepatitis C Virus antibody ELISA Kit
- Catalogue No.
- EH3774
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- HRP indrect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 2 hours
- Range
- Qualitative
- Sensitivity
- Qualitative
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with HCV-Ab , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
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- Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months 2-8°C for 12 months Average(%) 80 95-100 85-98