Human Cytokine Array C1000

Referência AAH-CYT-1000-8

Tamanho : 8SampleKit

Marca : RayBiotech

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Telefone : +1 850 650 7790

Human Cytokine Array C1000

RayBio® C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size2 Sample Kit, 4 Sample Kit, 8 Sample Kit
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected120
Recommended ApplicationsMultiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support
Membrane
Design PrincipleSandwich-based
Method Of DetectionChemiluminescence
Shipping TypeBlue ice
Storage-20°C

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
  • Gene Symbol: ADIPOQ
  • Gene ID: 9370
  • Accession #: Q15848
  • ELISA Kit: RayBio Human Acrp30 ELISA
AgRP
  • Gene Symbol: AgRP
  • Gene ID: 181
  • Accession #: O00253
  • ELISA Kit: RayBio Human AgRP ELISA
Amphiregulin
  • Gene Symbol: AREG
  • Gene ID: 374
  • Accession #: P15514
  • ELISA Kit: RayBio Human AREG ELISA
Angiogenin
  • Gene Symbol: ANG
  • Gene ID: 283
  • Accession #: P03950
  • ELISA Kit: RayBio Human Angiogenin ELISA
Angiopoietin-2
  • Gene Symbol: ANGPT2
  • Gene ID: 285
  • Accession #: O15123
  • ELISA Kit: RayBio Human ANGPT2 ELISA
Axl
  • Gene Symbol: Axl
  • Gene ID: 558
  • Accession #: P30530
  • ELISA Kit: RayBio Human Axl ELISA
BDNF
  • Gene Symbol: BDNF
  • Gene ID: 627
  • Accession #: P23560
  • ELISA Kit: RayBio Human BDNF ELISA
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 2247
  • Accession #: P09038
  • ELISA Kit: RayBio Human bFGF ELISA
BLC
(CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: 10563
  • Accession #: O43927
  • ELISA Kit: RayBio Human BLC ELISA
BMP-4
  • Gene Symbol: BMP4
  • Gene ID: 652
  • Accession #: P12644
  • ELISA Kit: RayBio Human BMP-4 ELISA
BMP-6
  • Gene Symbol: BMP6
  • Gene ID: 654
  • Accession #: P22004
  • ELISA Kit: RayBio Human BMP-6 ELISA
beta-NGF
  • Gene Symbol: NGF
  • Gene ID: 4803
  • Accession #: P01138
  • ELISA Kit: RayBio Human beta-NGF ELISA
Betacellulin
(BTC)
  • Gene Symbol: BTC
  • Gene ID: 685
  • Accession #: P35070
  • ELISA Kit: RayBio Human BTC ELISA
CCL28
(MEC)
  • Gene Symbol: CCL28
  • Gene ID: 56477
  • Accession #: Q9NRJ3
  • ELISA Kit: RayBio Human CCL28 ELISA
CK beta 8-1
(CCL23)
  • Gene Symbol: CCL23
  • Gene ID: 6368
  • Accession #: P55773
  • ELISA Kit: RayBio Human Ckbeta8 ELISA
CNTF
  • Gene Symbol: CNTF
  • Gene ID: 1270
  • Accession #: P26441
  • ELISA Kit: RayBio Human CNTF ELISA
CTACK
(CCL27)
  • Gene Symbol: CCL27
  • Gene ID: 10850
  • Accession #: Q9Y4X3
  • ELISA Kit: RayBio Human CTACK ELISA
Dtk
  • Gene Symbol: TYRO3
  • Gene ID: 7301
  • Accession #: Q06418
  • ELISA Kit: RayBio Human Dtk ELISA
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
  • ELISA Kit: RayBio Human EGF ELISA
EGFR
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
  • ELISA Kit: RayBio Human EGFR ELISA
ENA-78
(CXCL5)
  • Gene Symbol: CXCL5
  • Gene ID: 6374
  • Accession #: P42830
  • ELISA Kit: RayBio Human ENA-78 ELISA
Eotaxin-1
(CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
  • ELISA Kit: RayBio Human Eotaxin-1 ELISA
Eotaxin-2
(MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: 6369
  • Accession #: O00175
  • ELISA Kit: RayBio Human Eotaxin-2 ELISA
Eotaxin-3
(CCL26)
  • Gene Symbol: CCL26
  • Gene ID: 10344
  • Accession #: Q9Y258
  • ELISA Kit: RayBio Human Eotaxin-3 ELISA
Fas
(TNFRSF6/Apo-1)
  • Gene Symbol: Fas
  • Gene ID: 355
  • Accession #: P25445
  • ELISA Kit: RayBio Human Fas ELISA
FGF-4
  • Gene Symbol: FGF4
  • Gene ID: 2249
  • Accession #: P08620
  • ELISA Kit: RayBio Human FGF-4 ELISA
FGF-6
  • Gene Symbol: FGF6
  • Gene ID: 2251
  • Accession #: P10767
  • ELISA Kit: RayBio Human FGF-6 ELISA
FGF-7
(KGF)
  • Gene Symbol: FGF7
  • Gene ID: 2252
  • Accession #: P21781
  • ELISA Kit: RayBio Human FGF-7 ELISA
FGF-9
  • Gene Symbol: FGF9
  • Gene ID: 2254
  • Accession #: P31371
  • ELISA Kit: RayBio Human FGF-9 ELISA
Flt-3 Ligand
  • Gene Symbol: FLT3LG
  • Gene ID: 2323
  • Accession #: P49771
  • ELISA Kit: RayBio Human Flt-3 L ELISA
Fractalkine
(CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: 6376
  • Accession #: P78423
  • ELISA Kit: RayBio Human Fractalkine ELISA
GCP-2
(CXCL6)
  • Gene Symbol: CXCL6
  • Gene ID: 6372
  • Accession #: P80162
  • ELISA Kit: RayBio Human GCP-2 ELISA
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
  • ELISA Kit: RayBio Human G-CSF ELISA
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
  • ELISA Kit: RayBio Human GDNF ELISA
GITR
(TNFRSF18)
  • Gene Symbol: TNFRSF18
  • Gene ID: 8784
  • Accession #: Q9Y5U5
  • ELISA Kit: RayBio Human GITR ELISA
GITR Ligand
(TNFSF18)
  • Gene Symbol: TNFSF18
  • Gene ID: 8995
  • Accession #: Q9UNG2
  • ELISA Kit: RayBio Human GITR L ELISA
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
  • ELISA Kit: RayBio Human GM-CSF ELISA
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: 2919
  • Accession #: P09341
  • ELISA Kit: RayBio Human GRO-alpha ELISA
HCC-4
(CCL16)
  • Gene Symbol: CCL16
  • Gene ID: 6360
  • Accession #: O15467
  • ELISA Kit: RayBio Human HCC-4 ELISA
HGF
  • Gene Symbol: HGF
  • Gene ID: 3082
  • Accession #: P14210
  • ELISA Kit: RayBio Human HGF ELISA
I-309
(TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
  • ELISA Kit: RayBio Human I-309 ELISA
ICAM-1
(CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
  • ELISA Kit: RayBio Human sICAM-1 ELISA
ICAM-3
(CD50)
  • Gene Symbol: ICAM3
  • Gene ID: 3385
  • Accession #: P32942
  • ELISA Kit: RayBio Human ICAM-3 ELISA
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
  • ELISA Kit: RayBio Human IFN-gamma ELISA
IGFBP-1
  • Gene Symbol: IGFBP1
  • Gene ID: 3484
  • Accession #: P08833
  • ELISA Kit: RayBio Human IGFBP-1 ELISA
IGFBP-2
  • Gene Symbol: IGFBP2
  • Gene ID: 3485
  • Accession #: P18065
  • ELISA Kit: RayBio Human IGFBP-2 ELISA
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: 3486
  • Accession #: P17936
  • ELISA Kit: RayBio Human IGFBP-3 ELISA
IGFBP-4
  • Gene Symbol: IGFBP4
  • Gene ID: 3487
  • Accession #: P22692
  • ELISA Kit: RayBio Human IGFBP-4 ELISA
IGFBP-6
  • Gene Symbol: IGFBP6
  • Gene ID: 3489
  • Accession #: P24592
  • ELISA Kit: RayBio Human IGFBP-6 ELISA
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
  • ELISA Kit: RayBio Human IGF-1 ELISA
IGF-1 R
  • Gene Symbol: IGF1R
  • Gene ID: 3480
  • Accession #: P08069
  • ELISA Kit: RayBio Human IGF-1 R ELISA
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Gene ID: 9173
  • Accession #: Q01638
  • ELISA Kit: RayBio Human IL-1 R4 ELISA
IL-1 R1
  • Gene Symbol: IL1R1
  • Gene ID: 3554
  • Accession #: P14778
  • ELISA Kit: RayBio Human IL-1 sRI ELISA
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
  • ELISA Kit: RayBio Human IL-10 ELISA
IL-11
  • Gene Symbol: IL11
  • Gene ID: 3589
  • Accession #: P20809
  • ELISA Kit: RayBio Human IL-11 ELISA
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 3593
  • Accession #: P29460
  • ELISA Kit: RayBio Human IL-12 p40 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
  • ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
  • ELISA Kit: RayBio Human IL-13 ELISA
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
  • ELISA Kit: RayBio Human IL-15 ELISA
IL-16
  • Gene Symbol: IL16
  • Gene ID: 3603
  • Accession #: Q14005
  • ELISA Kit: RayBio Human IL-16 ELISA
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
  • ELISA Kit: RayBio Human IL-17A ELISA
IL-1 alpha
(IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
  • ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
  • ELISA Kit: RayBio Human IL-1 beta ELISA
IL-1 ra
(IL-1 F3)
  • Gene Symbol: IL1RN
  • Gene ID: 3557
  • Accession #: P18510
  • ELISA Kit: RayBio Human IL-1 RA ELISA
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
  • ELISA Kit: RayBio Human IL-2 ELISA
IL-2 R alpha
  • Gene Symbol: IL2RA
  • Gene ID: 3559
  • Accession #: P01589
  • ELISA Kit: RayBio Human IL-2 R alpha ELISA
IL-3
  • Gene Symbol: IL3
  • Gene ID: 3562
  • Accession #: P08700
  • ELISA Kit: RayBio Human IL-3 ELISA
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
  • ELISA Kit: RayBio Human IL-4 ELISA
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
  • ELISA Kit: RayBio Human IL-5 ELISA
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
  • ELISA Kit: RayBio Human IL-6 ELISA
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: 3570
  • Accession #: P08887
  • ELISA Kit: RayBio Human IL-6 sR ELISA
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
  • ELISA Kit: RayBio Human IL-7 ELISA
IL-8
(CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
  • ELISA Kit: RayBio Human IL-8 ELISA
I-TAC
(CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: 6373
  • Accession #: O14625
  • ELISA Kit: RayBio Human I-TAC ELISA
Leptin
  • Gene Symbol: LEP
  • Gene ID: 3952
  • Accession #: P41159
  • ELISA Kit: RayBio Human Leptin ELISA
Light
(TNFSF14)
  • Gene Symbol: TNFSF14
  • Gene ID: 8740
  • Accession #: O43557
  • ELISA Kit: RayBio Human LIGHT ELISA
Lymphotactin
(XCL1)
  • Gene Symbol: XCL1
  • Gene ID: 6375
  • Accession #: P47992
  • ELISA Kit: RayBio Human Lympotactin  ELISA
MCP-1
(CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
  • ELISA Kit: RayBio Human MCP-1 ELISA
MCP-2
(CCL8)
  • Gene Symbol: CCL8
  • Gene ID: 6355
  • Accession #: P80075
  • ELISA Kit: RayBio Human MCP-2 ELISA
MCP-3
(MARC/CCL7)
  • Gene Symbol: CCL7
  • Gene ID: 6354
  • Accession #: P80098
  • ELISA Kit: RayBio Human MCP-3 ELISA
MCP-4
(CCL13)
  • Gene Symbol: CCL13
  • Gene ID: 6357
  • Accession #: Q99616
  • ELISA Kit: RayBio Human MCP-4 ELISA
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
  • ELISA Kit: RayBio Human M-CSF ELISA
MDC
(CCL22)
  • Gene Symbol: CCL22
  • Gene ID: 6367
  • Accession #: O00626
  • ELISA Kit: RayBio Human MDC ELISA
MIF
  • Gene Symbol: MIF
  • Gene ID: 4282
  • Accession #: P14174
  • ELISA Kit: RayBio Human MIF ELISA
MIG
(CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
  • ELISA Kit: RayBio Human MIG ELISA
MIP-1 alpha
(CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
  • ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta
(CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
  • ELISA Kit: RayBio Human MIP-1 beta ELISA
MIP-1 delta
(CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
  • ELISA Kit: RayBio Human MIP-1 delta ELISA
MIP-3 alpha
(CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
  • ELISA Kit: RayBio Human MIP-3 alpha ELISA
MIP-3 beta
(CCL19)
  • Gene Symbol: CCL19
  • Gene ID: 6363
  • Accession #: Q99731
  • ELISA Kit: RayBio Human MIP-3 beta ELISA
MSP alpha/beta
  • Gene Symbol: MST1
  • Gene ID: 4485
  • Accession #: P26927
  • ELISA Kit: RayBio Human MSP ELISA
NAP-2
(PPBP/CXCL7)
  • Gene Symbol: Ppbp
  • Gene ID: 5473
  • Accession #: P02775
  • ELISA Kit: RayBio Human NAP-2 ELISA
NT-3
  • Gene Symbol: NTF3
  • Gene ID: 4908
  • Accession #: P20783
  • ELISA Kit: RayBio Human NT-3 ELISA
NT-4
  • Gene Symbol: NTF4
  • Gene ID: 4909
  • Accession #: P34130
  • ELISA Kit: RayBio Human NT-4 ELISA
Oncostatin M
  • Gene Symbol: OSM
  • Gene ID: 5008
  • Accession #: P13725
  • ELISA Kit: RayBio Human OSM ELISA
Osteoprotegerin
(TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Gene ID: 4982
  • Accession #: O00300
  • ELISA Kit: RayBio Human OPG ELISA
PARC
(CCL18)
  • Gene Symbol: CCL18
  • Gene ID: 6362
  • Accession #: P55774
  • ELISA Kit: RayBio Human PARC ELISA
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
  • ELISA Kit: RayBio Human PDGF-BB ELISA
PLGF
  • Gene Symbol: PGF
  • Gene ID: 5228
  • Accession #: P49763
  • ELISA Kit: RayBio Human PLGF ELISA
RANTES
(CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
  • ELISA Kit: RayBio Human RANTES ELISA
SCF
  • Gene Symbol: KITLG
  • Gene ID: 4254
  • Accession #: P21583
  • ELISA Kit: RayBio Human SCF ELISA
SDF-1 alpha
(CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Gene ID: 6387
  • Accession #: P48061
  • ELISA Kit: RayBio Human SDF-1 alpha ELISA
gp130
  • Gene Symbol: Il6st
  • Gene ID: 3572
  • Accession #: P40189
  • ELISA Kit: RayBio Human sgp130 ELISA
TNF RII
(TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: 7133
  • Accession #: P20333
  • ELISA Kit: RayBio Human sTNFRII ELISA
TNF RI
(TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 7132
  • Accession #: P19438
  • ELISA Kit: RayBio Human sTNFRI ELISA
TARC
(CCL17)
  • Gene Symbol: CCL17
  • Gene ID: 6361
  • Accession #: Q92583
  • ELISA Kit: RayBio Human TARC ELISA
TECK
(CCL25)
  • Gene Symbol: CCL25
  • Gene ID: 6370
  • Accession #: O15444
  • ELISA Kit: RayBio Human TECK ELISA
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
  • ELISA Kit: RayBio Human TGF-beta 1 ELISA
Thrombopoietin
(TPO)
  • Gene Symbol: THPO
  • Gene ID: 7066
  • Accession #: P40225
  • ELISA Kit: RayBio Human TPO ELISA
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
  • ELISA Kit: RayBio Human TIMP-1 ELISA
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
  • ELISA Kit: RayBio Human TIMP-2 ELISA
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
  • ELISA Kit: RayBio Human TNF-alpha ELISA
TNF beta
(TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
  • ELISA Kit: RayBio Human TNF-beta ELISA
TRAIL R3
(TNFRSF10C)
  • Gene Symbol: TNFRSF10C
  • Gene ID: 8794
  • Accession #: O14798
  • ELISA Kit: RayBio Human TRAIL R3 ELISA
TRAIL R4
(TNFRSF10D)
  • Gene Symbol: TNFRSF10D
  • Gene ID: 8793
  • Accession #: Q9UBN6
  • ELISA Kit: RayBio Human TRAIL R4 ELISA
uPAR
  • Gene Symbol: PLAUR
  • Gene ID: 5329
  • Accession #: Q03405
  • ELISA Kit: RayBio Human uPAR ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
  • ELISA Kit: RayBio Human VEGF-A ELISA
VEGF-D
  • Gene Symbol: FIGF
  • Gene ID: 2277
  • Accession #: O43915
  • ELISA Kit: RayBio Human VEGF-D ELISA

Typical Data

RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to profile secreted proteins from LILRB4+ versus LILRB4-knockout AML cells, revealing reduced uPAR expression upon LILRB4 deletion. Figure adapted from Deng et al., Nature, 2018.


RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to compare secreted proteins from ghMSCs and hMSCs, revealing higher levels of IL-8, GRO chemokines, MCP-1, VEGF, IGFBP-4, and HGF in ghMSCs. Figure adapted from Lee et al., Molecular Therapy, 2025.


RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to compare secreted proteins from untreated and cisplatin-treated cancer-associated fibroblasts (CAFs), revealing increased secretion of HGF, IL-1α, MCP-1, SDF-1, and angiogenin in response to chemotherapy. Figure adapted from Palliyage et al., Lung Cancer, 2023.


RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to profile TNF-α–induced chemokine secretion in triple-negative breast cancer cells, revealing that thymoquinone reduced CCL2, CCL3, CCL4, and CCL20 expression. Figure adapted from Adinew et al., International Journal of Molecular Sciences, 2023.


RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to assess cytokine secretion from macrophages cultured on titanium surfaces with varying coatings, revealing surface-dependent changes in immune signaling. Figure adapted from Huang et al., Bioactive Materials, 2023.

Kit Components

  • Human Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual

Additional Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Excel (AAH-CYT-6_Analysis_Tool.xls)
Excel (AAH-CYT-7_Analysis_Tool.xls)

citations

  • Circulating Metabolites Treat Human TMJ-OA by Eliminating Senescent Chondrocytes via the C1QBP/C1q/p14ARF Axis

    Species:
    Human
    Sample Type:
    Other Body Fluid (TMJ synovial fluid)
    Year:
    2026
    Journal Name:
    Journal of Extracellular Vesicles
    PubMed ID:
    41619174
  • Targeting ABL kinases overcomes melanoma immune evasion following resistance to MAPK inhibition

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (conditioned media from serum-starved, drug-treated, or transfected cell lines)
    Year:
    2026
    Journal Name:
    Nature Communications
    PubMed ID:
    42764300
  • Tumor neoantigen gene C7orf50 remodels the immune microenvironment by recruiting tumor-associated macrophages to promote hepatocellular carcinoma progression and lung metastasis

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (HCC cell culture supernatant)
    Year:
    2026
    Journal Name:
    Journal for Immunotherapy of Cancer
    PubMed ID:
    41592889
  • Novel stem cell therapy for cerebral palsy using stem cells from human exfoliated deciduous teeth

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (NSC-conditioned medium from co-culture with SHED, BMMSC or DF)
    Year:
    2026
    Journal Name:
    Stem Cell Research & Therapy
    PubMed ID:
    41578408
  • Nanobioreactor detection of space-associated hematopoietic stem and progenitor cell aging

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (nanobioreactor culture supernatants)
    Year:
    2025
    Journal Name:
    Cell Stem Cell
    PubMed ID:
    40912236
  • Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly

    Species:
    Human
    Sample Type:
    Other / Unspecified (cytokines secreted by ADSCs)
    Year:
    2025
    Journal Name:
    Cell Death & Disease
    PubMed ID:
    40595500
  • GPRC5A/CXCL8/NLRP3-mediated neutrophil extracellular traps drive gemcitabine-nab-paclitaxel resistance in pancreatic adenocarcinoma

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