Human CD13 Antibody : PE
Referência OASB01047
Tamanho : 100tests
Marca : Aviva Systems Biology
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Human CD13 Antibody : PE (OASB01047)
| Datasheets/Manuals | Printable datasheet for Human CD13 Antibody : PE (OASB01047) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | 22A5 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | R-PE |
| Application | FC |
| Additional Information | Description: CD13, also known as aminopeptidase N, is a 150 kDa type II integral membrane glycoprotein. It is expressed on granulocytes, monocytes and their precursors, and on mast cells. CD13 is a zinc-binding metalloprotease that is involved in cell surface antigen presentation via its digestion of many MHC-bound regulatory peptides. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Osteoclasts from osteoclastomas |
| Concentration | Lot specific |
| Specificity | CD13 |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein conjugate R-phycoerythrin conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, OxfordUniversity Press, Oxford 2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD13 Section, Academic Press, New York, p. 166 4. Bradstock, K.F., E.J. Favaloro, A. Kabral, A. Kerr, W.G. Hughes, and E. Musgrove 1985. Brit. J. Haematol. 61:11 5. Dang, N.H., Y. Torimoto, K. Shiamamura, T. Tanaka, J.F. Daley, S.F. Schlossman, and C. Morimoto. 1991. J. Immunol. 147:2825 6. Larsen, S.L., L.O. Pedersen, S. Buus, and A. Stryhn. 1996. J. Exp. Med. 184:183. |
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| Gene Symbol | ANPEP |
| Gene Full Name | Aminopeptidase N |
| Alias Symbols | APN, CD13, LAP1, P150, PEPN, GP150 |
| NCBI Gene Id | 290 |
| Protein Name | aminopeptidase N |
| Description of Target | Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. |
| Uniprot ID | P15144 |
| Protein Accession # | NP_001141.2 |
| Nucleotide Accession # | NM_001150.2 |
